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JK9-3d yeast strain酵母菌株
JK9-3d There are a, alpha and a/alpha diploids of JK9-3d with the following genotypes: Genotypes: JK9-3da MATa leu2-3,112 ura3-52 rme1 trp1 his4 JK9-3dα has the same genotype as JK9-3da with the exception of the MAT locus JK9-3da/α is homozygous for all markers except mating type Notes: JK9-3d was constructed by Jeanette Kunz while in Mike Hall's lab. She made the original strain while Joe Heitman isolated isogenic strains of opposite mating type and derived the a/alpha isogenic diploid by mating type switching. It has in its background S288c, a strain from the Oshima lab, and a strain from the Herskowitz lab. It was chosen because of its robust growth and sporulation, as well as good growth on galactose (GAL+) (so that genes under control of the galactose promoter could be induced). It may also have a SUP mutation that allows translation through premature STOP codons and therefore produces functional alleles with many point mutations. Recent work shows that JK9-3d carries an rme1 mutation that may be responsible for the rapid G1 arrest of this strain upon exposure to rapamycin (Moreno-Torres M, et al. (2015) Nat Commun 6:8256) References: Heitman et al. (1991a) Science 253(5022):905-9 and Heitman et al. (1991b) Proc Natl Acad Sci U S A 88(5):1948-52
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JK9-3d There are a, alpha and a/alpha diploids of JK9-3d with the following genotypes: Genotypes: JK9-3da MATa leu2-3,112 ura3-52 rme1 trp1 his4 JK9-3dα has the same genotype as JK9-3da with the exception of the MAT locus JK9-3da/α is homozygous for all markers except mating type Notes: JK9-3d was constructed by Jeanette Kunz while in Mike Hall's lab. She made the original strain while Joe Heitman isolated isogenic strains of opposite mating type and derived the a/alpha isogenic diploid by mating type switching. It has in its background S288c, a strain from the Oshima lab, and a strain from the Herskowitz lab. It was chosen because of its robust growth and sporulation, as well as good growth on galactose (GAL+) (so that genes under control of the galactose promoter could be induced). It may also have a SUP mutation that allows translation through premature STOP codons and therefore produces functional alleles with many point mutations. Recent work shows that JK9-3d carries an rme1 mutation that may be responsible for the rapid G1 arrest of this strain upon exposure to rapamycin (Moreno-Torres M, et al. (2015) Nat Commun 6:8256) References: Heitman et al. (1991a) Science 253(5022):905-9 and Heitman et al. (1991b) Proc Natl Acad Sci U S A 88(5):1948-52
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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