BacterioMatch II Two-Hybrid System Vector细菌双杂交系统质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

BacterioMatch II Two-Hybrid System Vector细菌双杂交系统质粒载体

Our exclusive BacterioMatch II two-hybrid system is an efficient method for detecting protein-protein interactions in vivo. The BacterioMatch II twohybrid system is based on a methodology developed by Dove, Joung, and Hochschild of Harvard Medical School and further refined by Joung and Pabo of the Massachusetts Institute of Technology.1, 2, 3 This E. coli-based system offers advantages over our original BacterioMatch two-hybrid system. Featuring a new HIS3-aadA reporter cassette, the BacterioMatch II system offers the ability to screen libraries for harder-to-find binding partners with reduced background. Detection of protein-protein interactions is based on transcriptional activation of the HIS3 reporter gene, which allows growth in the presence of 3-amino-1,2,4-triazole (3-AT), a competitive inhibitor of His3 enzyme. Positives are verified by using the aadA gene, which confers streptomycin resistance, as a secondary reporter. The BacterioMatch II Two-Hybrid System Vector Kit (Catalog #240065) is designed for the characterization of protein-protein interactions between a pair of proteins cloned in the pBT (bait) and the pTRG (target) vectors. Once an interaction pair has been identified, the system may be used to map the interaction domain of either the bait or target protein after site-directed mutagenesis. For two-hybrid screening using the pBT-bait plasmid to identify proteinprotein interaction partners from a pTRG cDNA plasmid library, we offer the BacterioMatch II Two-Hybrid System Library Construction Kit (Stratagene Catalog #200412) and the BacterioMatch II Two-Hybrid System Library Construction Kit with Electrocompetent Cells

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