pGAPZα A, B, C vectors毕赤酵母组成型表达质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心
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- 货 号：pGAPZα A, B, and C
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pGAPZα A, B, C vectors毕赤酵母组成型表达质粒载体
Introduction The glyceraldehyde-3-phosphate dehydrogenase (GAPDH) enzyme is constitutively expressed at high levels in many organisms, including Pichia pastoris. The promoter of the gene (GAP) encoding the GAPDH protein has recently been characterized and shown to express recombinant proteins to high levels in Pichia pastoris, depending on the carbon source used (Waterham et al., 1997). The level of expression seen with the GAP promoter (PGAP) can be slightly higher than that obtained with the AOX1 promoter. The pGAPZ A, B, and C vectors (2.9 kb) and pGAPZα A, B, and C (3.1 kb) vectors use the GAP promoter to constitutively express recombinant proteins in Pichia pastoris. Proteins can be expressed as fusions to a C-terminal peptide containing the myc epitope for detection and a polyhistidine tag for purification on metal-chelating resin (i.e. ProBond™). In addition, pGAPZα produces proteins fused to an N-terminal peptide encoding the Saccharomyces cerevisiae α-factor secretion signal. Both vectors are supplied in three reading frames to facilitate in frame cloning with the C-terminal tag and/or the N-terminal secretion signal.Selection of these vectors is based on the dominant selectable marker, Zeocin™,which is bifunctional in both Pichia and E. coli.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
Introduction The glyceraldehyde-3-phosphate dehydrogenase (GAPDH) enzyme is constitutively expressed at high levels in many organisms, including Pichia pastoris. The promoter of the gene (GAP) encoding the GAPDH protein has recently been characterized and shown to express recombinant proteins to high levels in Pichia pastoris, depending on the carbon source used (Waterham et al., 1997). The level of expression seen with the GAP promoter (PGAP) can be slightly higher than that obtained with the AOX1 promoter. The pGAPZ A, B, and C vectors (2.9 kb) and pGAPZα A, B, and C (3.1 kb) vectors use the GAP promoter to constitutively express recombinant proteins in Pichia pastoris. Proteins can be expressed as fusions to a C-terminal peptide containing the myc epitope for detection and a polyhistidine tag for purification on metal-chelating resin (i.e. ProBond™). In addition, pGAPZα produces proteins fused to an N-terminal peptide encoding the Saccharomyces cerevisiae α-factor secretion signal. Both vectors are supplied in three reading frames to facilitate in frame cloning with the C-terminal tag and/or the N-terminal secretion signal.Selection of these vectors is based on the dominant selectable marker, Zeocin™,which is bifunctional in both Pichia and E. coli.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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