184B5 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

184B5 cell line细胞株

Organism Homo sapiens, human Tissue mammary gland/breast: epithelium Cell Type chemically transformed Product Format frozen Morphology epithelial Culture Properties adherent Biosafety Level 1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. Disease normal Age 21 years Gender female
Karyotype 47, XX Derivation Cells derived from the tissue were exposed to benzo(a)pyrene, and a transformed line was established. The 184B5 cell line was established from normal mammary tissue obtained from a normal reduction mammoplasty. Clinical Data female Tumorigenic No Effects No, the cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium. Comments The line appears to be immortal, but is not malignant. When seeded at low density, the cells grow in tightly packed colonies.
Complete Growth Medium The base medium for this cell line (MEBM) along with the additives can be obtained from Lonza/Clonetics Corporation as a kit: MEGM, Kit Catalog No. CC-3150. ATCC does not use the GA-1000 (gentamycin-amphotericin B mix) provided with kit. To make the complete growth medium, you will need to add the following components to the kit (sold separately): 1 ng/ml cholera toxin Note: Do not filter complete medium Subculturing Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximately 125 x g for 5 to 10 minutes. Discard supernatant and resuspend cells in fresh serum-free growth medium. Add appropriate aliquots of cell suspension to new culture vessels.
U.S. Patent Number 4,808,532 Name of Depositor The United States of America U.S. Patent Number 4,808,532U.S. Patent Number 4,808,532 Name of Depositor The United States of America U.S. Patent Number 4,808,532 Place culture vessels in incubators at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended Medium Renewal: Every 2 to 3 days Cryopreservation Freeze medium: Complete growth medium suplemented with 10% (v/v) fetal bovine serum and 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase Note: Lots manufactured prior to 05/20/2020 may have used a different cryopreservative, contact customer service if needed. Culture Conditions Temperature: 37°C

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