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pADL-22C噬菌体展示载体质粒plasmid
Technical Description pADL™-22c is a phagemid vector designed for phage display on the N-terminal side of the protein III of the filamentous bacteriophage M13 or equivalent. This vector contains a PelB leader sequence for expression in the periplasm, a double-SfiI cloning site to introduce scFvs or Fab fragments, a HIS tag for purification, a HA tag for detection and an amber codon located before the full-length copy of the gene III sequence. On non-suppressive bacterial strains, free scFvs or Fab fragments are produced in the periplasm where they can be assayed for binding or purified for further testing. Expression of the fusion is under the control of a lac promoter that has been adjusted at a level comparable to the widely used phagemid pCOMB3.Applications Phage display at the N-terminal side of gene III protein of the filamentous bacteriophage. Free scFv or Fab expression.Specifications General Characteristics: Plasmid Size: 3957 Promoter: lac promoter Leader Peptide: PelB Cloning Site: double-SfiI/BglI, NotI-SpeI Purification: HIS tag Detection: HA tag Fusion Protein: full length gene III protein and conditional Amber stop codon Selection: ampicillin Replication: oriF1, pMB1 Physical Characteristics: Concentration: 0.5 µg/µl. Product Size: 10 µg. Buffer: DNA Conservation Buffer (Tris/HCL 5 mM, EDTA 0.1 mM, pH 8.5, sterile). Storage Temperature: -20°C. Quality Control & Certification of Analysis Product Size: Digestion by EcoRI and NheI generates 2 fragments of 2.6 kb and 1.4 kb. Product Sequence: Complete vector sequence is analyzed. Certification: Product meets all specifications.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
Technical Description pADL™-22c is a phagemid vector designed for phage display on the N-terminal side of the protein III of the filamentous bacteriophage M13 or equivalent. This vector contains a PelB leader sequence for expression in the periplasm, a double-SfiI cloning site to introduce scFvs or Fab fragments, a HIS tag for purification, a HA tag for detection and an amber codon located before the full-length copy of the gene III sequence. On non-suppressive bacterial strains, free scFvs or Fab fragments are produced in the periplasm where they can be assayed for binding or purified for further testing. Expression of the fusion is under the control of a lac promoter that has been adjusted at a level comparable to the widely used phagemid pCOMB3.Applications Phage display at the N-terminal side of gene III protein of the filamentous bacteriophage. Free scFv or Fab expression.Specifications General Characteristics: Plasmid Size: 3957 Promoter: lac promoter Leader Peptide: PelB Cloning Site: double-SfiI/BglI, NotI-SpeI Purification: HIS tag Detection: HA tag Fusion Protein: full length gene III protein and conditional Amber stop codon Selection: ampicillin Replication: oriF1, pMB1 Physical Characteristics: Concentration: 0.5 µg/µl. Product Size: 10 µg. Buffer: DNA Conservation Buffer (Tris/HCL 5 mM, EDTA 0.1 mM, pH 8.5, sterile). Storage Temperature: -20°C. Quality Control & Certification of Analysis Product Size: Digestion by EcoRI and NheI generates 2 fragments of 2.6 kb and 1.4 kb. Product Sequence: Complete vector sequence is analyzed. Certification: Product meets all specifications.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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