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SKOV3/DDP cell line耐药细胞株
The SKOV3 cell line is a human OC adenocarcinoma cell line, and SKOV3/DDP is a cisplatin-resistant variant of SKOV3Organism Homo sapiens, human Tissue ovary: ascites Product Format frozen Morphology epithelial Culture Properties adherent Biosafety Level 1 Disease adenocarcinoma Age 64 years Gender female Ethnicity Caucasian Applications This cell line is a suitable transfection host. Karyotype This is a hypodiploid human cell line. The modal chromosome number was 43, occurring in 63.3% of cells. The range was 42 to 45. The rate of higher ploidies was 32%. The del(1)(q21), der(13)t(1;?;13) (q11;?;q34), der(11)t(11;?) (q12), del(10)(q22) and 3 other marker chromosomes were common to most cells, and 3 others were found only in some cells. One N11 had the HSR segment from p11 to the distal end. The normal N10, N12, N15, N17 and N19 were absent. Others were either single or paired. There were from 1 to 6 rearranged and unassignable chromosomes. The X chromosome was either single or paired. Images Clinical Data 64 years Caucasian female Antigen Expression Blood Type B; Rh+ Genes Expressed Blood Type B; Rh+ Tumorigenic Yes Effects Yes, in nude mice; forms moderately well differentiated adenocarcinoma consistent with ovarian primary Comments SK-OV-3 cells are resistant to tumor necrosis factor and to several cytotoxic drugs including diphtheria toxin, cis-platinum and adriamycin. Complete Growth Medium The base medium for this cell line is McCoy's 5a Medium Modified. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Subculturing Protocol: 1.Remove and discard culture medium. 2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. 3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product. 4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. 5.Add appropriate aliquots of the cell suspension to new culture vessels. 6.Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended Medium Renewal: 2 to 3 times per week Cryopreservation Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase Culture Conditions Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
The SKOV3 cell line is a human OC adenocarcinoma cell line, and SKOV3/DDP is a cisplatin-resistant variant of SKOV3Organism Homo sapiens, human Tissue ovary: ascites Product Format frozen Morphology epithelial Culture Properties adherent Biosafety Level 1 Disease adenocarcinoma Age 64 years Gender female Ethnicity Caucasian Applications This cell line is a suitable transfection host. Karyotype This is a hypodiploid human cell line. The modal chromosome number was 43, occurring in 63.3% of cells. The range was 42 to 45. The rate of higher ploidies was 32%. The del(1)(q21), der(13)t(1;?;13) (q11;?;q34), der(11)t(11;?) (q12), del(10)(q22) and 3 other marker chromosomes were common to most cells, and 3 others were found only in some cells. One N11 had the HSR segment from p11 to the distal end. The normal N10, N12, N15, N17 and N19 were absent. Others were either single or paired. There were from 1 to 6 rearranged and unassignable chromosomes. The X chromosome was either single or paired. Images Clinical Data 64 years Caucasian female Antigen Expression Blood Type B; Rh+ Genes Expressed Blood Type B; Rh+ Tumorigenic Yes Effects Yes, in nude mice; forms moderately well differentiated adenocarcinoma consistent with ovarian primary Comments SK-OV-3 cells are resistant to tumor necrosis factor and to several cytotoxic drugs including diphtheria toxin, cis-platinum and adriamycin. Complete Growth Medium The base medium for this cell line is McCoy's 5a Medium Modified. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Subculturing Protocol: 1.Remove and discard culture medium. 2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. 3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product. 4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. 5.Add appropriate aliquots of the cell suspension to new culture vessels. 6.Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended Medium Renewal: 2 to 3 times per week Cryopreservation Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase Culture Conditions Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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