AAV pCAG-FLEX-mRuby3-WPRE plasmid 腺相关病毒表达质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

AAV pCAG-FLEX-mRuby3-WPRE plasmid 腺相关病毒表达质粒载体

Can be used to generate AAV virus that will express mRuby3 in the presence of Cre
Backbone size w/o insert (bp)5723 Total vector size (bp)6455 Modifications to backboneA fragment containing reverse-complemented mRuby3 was swapped into replace tdTomato. Total AAV packaging size (including ITRs and DNA in between) is 3858 bp. Vector typeAAV, Cre/Lox GROWTH IN BACTERIA Bacterial Resistance(s)Ampicillin Growth Temperature37°C Growth Strain(s)NEB Stable Copy numberUnknown GENE/INSERT Gene/Insert namemRuby3 Alt namemonomeric ruby3 Alt namemRuby3 red fluorescent protein SpeciesSynthetic Insert Size (bp)733 CLONING INFORMATION Cloning methodRestriction Enzyme 5′ cloning siteAscI (not destroyed) 3′ cloning siteFseI (not destroyed) 5′ sequencing primergcaacgtgctggttattgtg 3′ sequencing primerTAGAAGGCACAGTCGAGG

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