首页 » pWPT-GFP plasmid慢病毒基因重组荧光质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pWPT-GFP plasmid慢病毒基因重组荧光质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

  • 价  格:¥49865
  • 货  号:pWPT-GFP plasmid
  • 产  地:北京
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pWPT-GFP plasmid慢病毒基因重组荧光质粒载体

pWPT or pWPXL can be used for constitutive transgene expression. pWPXL contains the EF-1alpha promoter + intron that gives you high expression, as RNA loves to be spliced (it goes more efficiently out of the nucleus). pWPT contains only the EF-1alpha promoter The loxP site in the 3'LTR is duplicated to the 5'LTR during reverse transcription in the target cells. This allows for removal (if necessary) of an integrated provirus by Cre. Unique restriction sites at key positions will allow you to change promoter and transgene. Please note that ClaI in this vector is blocked by Dam methylation. This plasmid needs to be grown in a Dam- bacteria strain if you wish to use ClaI for cloning.

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