NTHY-ORI3-1 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
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- 货 号：NTHY-ORI3-1
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NTHY-ORI3-1 cell line细胞株
Cell Line Name: Nthy-ori 3-1 Keywords: Human thyroid follicular epithelial Cell Line Description: Normal human primary thyroid follicular epithelial cells were transfected with a plasmid containing an origin-defective SV40 genome (SV-ori) to immortalise them. SV40 DNA was able to immortalise normal thyroid epithelial cells with the retention of several different functions. Species: Human Tissue of Origin: Thyroid CellType: Epithelial Growth Mode: Adherent DNA Profile: STR-PCR Data: Amelogenin: X CSF1PO: 12 D13S317: 11 D16S539: 12,13 D5S818: 11 D7S820: 7,12 THO1: 7 TPOX: 9 vWA: 16,18 Image Karyotype: Not specified GMO Status: Genetically Modified Organism Class 1 (GMO1) Subculture Routine: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C. Culture Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). Originator: Yes Country: UK References: Lemoine NR, Mayall ES, Jones T, Sheer D, McDermid S, Kendall-Taylor P, Wynford-Thomas D 1989 Characterisation of human thyroid epithelial cells immortalised in vitro by simian virus 40 DNA transfection. Br J Cancer. 60(6):897-903 PMID: 2557880.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
Cell Line Name: Nthy-ori 3-1 Keywords: Human thyroid follicular epithelial Cell Line Description: Normal human primary thyroid follicular epithelial cells were transfected with a plasmid containing an origin-defective SV40 genome (SV-ori) to immortalise them. SV40 DNA was able to immortalise normal thyroid epithelial cells with the retention of several different functions. Species: Human Tissue of Origin: Thyroid CellType: Epithelial Growth Mode: Adherent DNA Profile: STR-PCR Data: Amelogenin: X CSF1PO: 12 D13S317: 11 D16S539: 12,13 D5S818: 11 D7S820: 7,12 THO1: 7 TPOX: 9 vWA: 16,18 Image Karyotype: Not specified GMO Status: Genetically Modified Organism Class 1 (GMO1) Subculture Routine: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C. Culture Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). Originator: Yes Country: UK References: Lemoine NR, Mayall ES, Jones T, Sheer D, McDermid S, Kendall-Taylor P, Wynford-Thomas D 1989 Characterisation of human thyroid epithelial cells immortalised in vitro by simian virus 40 DNA transfection. Br J Cancer. 60(6):897-903 PMID: 2557880.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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