Hep-55.1c cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

Hep-55.1c cell line细胞株

Origin and General Characteristics Organism: Mouse (Mus musculus) Strain: C57BL/6J Age/Stage: Adult Gender: Female Tissue: Liver Morphology: Epithelial Cell type: Hepatoma Growth Properties: Monolayer, adherent Description: Established from the primary hepatocellular carcinoma of C57BL/6J mice. References: Kress S et al. p53 mutations are absent from carcinogen-induced mouse liver tumors but occur in cell lines established from these tumors. Molecular carcinogenesis 6: 148-158,1992. Culture Conditions and Handling Culture Medium: DMEM supplemented with 4.5 g/L glucose, 2 mM L-glutamine and 10% fetal bovine serum. Culturing process: Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks).Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3-5 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium. Split Ratio: A ratio of 1:4 to 1:8 is recommended Fluid Renewal: Every 3 to 5 days Freeze Medium: CM-1 Freezing Recovery: Start culture from cryovial at a cell density of 3-4x104 cells/cm2. The cells will recover within 24-48 hrs. Sterility: Fluorescence (DAPI) test: negative; Mycoplasma specific PCR: negative; Bacteria specific PCR: negative Biosafety Level: 1 Special Features of the Cell Line Tumorigenic: yes, in C57BL/6J mice Viruses: SMRV: Negative, as confirmed by Real-Time PCR Authentication: The mouse origin was verified by Real-Time PCR. Protein pattern: Keratin 8, keratin 18, Vimentin Mutations: p53 wt

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