Rotavirus A strain M37轮状病毒 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥1998660
- 货 号：Rotavirus A strain M37
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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Rotavirus A strain M37轮状病毒
Classification Reoviridae, Rotavirus, Rotavirus A，P6 type Deposited As Human rotavirus, strain M37 Agent Rotavirus A Strain M37 Applications Enteric Research Biosafety Level 2 Recommended Host MA-104 Clone 1 Growth Conditions Temperature: 37°C Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 98-100% confluent. Calculate volume of virus seed required to inoculate with an optimal MOI (e.g. 0.2-0.4). Activate virus by incubating in 10 µg/mL Trypsin 1:250 within a 37°C water bath for 30 minutes. If needed, combine activated virus with enough virus growth medium to inoculate with a small amount of inoculum (e.g. 1 mL per 25 cm2). Wash cell monolayer twice with DPBS or EMEM. Adsorb activated virus for 1-2 hours at 37°C, in a humidified 5% CO2 environment with slow continuous rocking. End adsorption by removing the inoculum and then adding virus growth medium. Incubation: 12 days Description: Rotavirus vaccine strain M37 (serotype 1), recovered from the stool of an asymptomatic newborn infant and serially passaged in cell culture, was given orally to adults, children, and infants. Serologic responses were detected by neutralization assay or EIA in 59% of 17 adults (10(5)-pfu dose), 55%-60% of 21 infants and children (10(4)-pfu dose), and 70% of 10 infants (10(5)-pfu dose), vaccine virus was shed by 24%, 20%-36%, and 70%, respectively. In adults, neutralizing antibody rises to strain M37 and the related serotype 1 strain Wa occurred with equal frequency (41% vs. 47%). In pediatric subjects, the former were more frequent (36%-40%) than the latter (10%-18%). This was also true of 8 infants who received two doses of vaccine. Mild gastrointestinal illnesses occurred with equal frequency in pediatric subjects who received vaccine or placebo. Thus, strain M37 was well tolerated and immunogenic in young infants, but elicited primarily vaccine-strain-specific rather than serotype-specific neutralizing antibody responses.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
Classification Reoviridae, Rotavirus, Rotavirus A，P6 type Deposited As Human rotavirus, strain M37 Agent Rotavirus A Strain M37 Applications Enteric Research Biosafety Level 2 Recommended Host MA-104 Clone 1 Growth Conditions Temperature: 37°C Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 98-100% confluent. Calculate volume of virus seed required to inoculate with an optimal MOI (e.g. 0.2-0.4). Activate virus by incubating in 10 µg/mL Trypsin 1:250 within a 37°C water bath for 30 minutes. If needed, combine activated virus with enough virus growth medium to inoculate with a small amount of inoculum (e.g. 1 mL per 25 cm2). Wash cell monolayer twice with DPBS or EMEM. Adsorb activated virus for 1-2 hours at 37°C, in a humidified 5% CO2 environment with slow continuous rocking. End adsorption by removing the inoculum and then adding virus growth medium. Incubation: 12 days Description: Rotavirus vaccine strain M37 (serotype 1), recovered from the stool of an asymptomatic newborn infant and serially passaged in cell culture, was given orally to adults, children, and infants. Serologic responses were detected by neutralization assay or EIA in 59% of 17 adults (10(5)-pfu dose), 55%-60% of 21 infants and children (10(4)-pfu dose), and 70% of 10 infants (10(5)-pfu dose), vaccine virus was shed by 24%, 20%-36%, and 70%, respectively. In adults, neutralizing antibody rises to strain M37 and the related serotype 1 strain Wa occurred with equal frequency (41% vs. 47%). In pediatric subjects, the former were more frequent (36%-40%) than the latter (10%-18%). This was also true of 8 infants who received two doses of vaccine. Mild gastrointestinal illnesses occurred with equal frequency in pediatric subjects who received vaccine or placebo. Thus, strain M37 was well tolerated and immunogenic in young infants, but elicited primarily vaccine-strain-specific rather than serotype-specific neutralizing antibody responses.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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