E-286 Murine leukemia virus嗜亲性小鼠白血病病毒VR-1350 BioVector NTCC质粒载体菌种细胞基因保藏中心

E-286 Murine leukemia virus嗜亲性小鼠白血病病毒VR-1350

Murine leukemia virus NTCC-AVR1350 Classification Retroviridae, Gammaretrovirus, Mammalian virus group Deposited As Moloney murine leukemia virus Agent Murine leukemia virus Strain E-286 Biosafety Level 2 Isolation biological clone of the NB-tropic component of Moloney murine lymphoid leukemia (NIH isolate) Comments Ecotropic (NB-tropic) MuLV See Hartley and Rowe (1975. J. Virol. 65:128) for information on propagation in SC-1 cells. Ecotropic (NB-tropic) MuLV. -70°C or lower Effect on Host Yes, in vitro effects: focus formation in mouse S(+)L(-) cells Foci formation in mouse S+L- cells; plaques on XC cells (ATCC CCL-165) Yes, in vitro effects: plaques on XC cells Recommended Host SC-1 cells (ATCC CRL-1404) mouse mouse cells in tissue culture Growth Conditions Incubation  10 days at 37°C in SC-1 cells (ATCC CRL-1404) Cryopreservation Storage temperature: -70C or lower Effect on Host Yes, in vitro effects: focus formation in mouse S(+)L(-) cells Foci formation in mouse S+L- cells; plaques on XC cells (ATCC CCL-165) Yes, in vitro effects: plaques on XC cells Source biological clone of the NB-tropic component of Moloney murine lymphoid leukemia (NIH isolate) References Bassin RH, et al. Abrogation of Fv-lb restriction with murine leukemia viruses inactivated by heat or by gamma irradiation. J. Virol. 26: 306-315, 1978. PubMed: 77908

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