Type-1 BAV Bovine adenovirus 1 牛腺病毒 BioVector NTCC质粒载体菌种细胞基因保藏中心
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- 货 号：BAV
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Type-1 BAV Bovine adenovirus 1 牛腺病毒
Bovine adenovirus 1 Cat No.:NTCC-AVR313 Classification Adenoviridae, Mastadenovirus, Bovine mastadenovirus A Deposited As Bovine adenovirus type 1 Agent Bovine adenovirus 1 Strain 10 Applications Respiratory research Biosafety Level 2 Isolation Feces of normal calf Product Format frozen 1.0 mL Images Comments REFERENCE STRAIN: Pereira HG et al. A short description of the Adenovirus group. Virology 20: 613-620, 1963. Agglutinates rat RBC. CPE may be minimal, frequently demonstrating less than 25% involvement of cell sheet, even with prolonged incubation. AAV reported to be present. Effect on Host CPE, cell rounding with vacuolization, clumping, and sloughing Recommended Host MDBK (NBL-1) cells (ATCC® CCL-22™) Growth Conditions Temperature: 37°C Recommendations for Infection: For best results, infection should be performed on a 70-80% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 4-10 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. Effect on Host CPE, cell rounding with vacuolization, clumping, and sloughing Source Feces of normal calf References Klein M, et al. Isolation from cattle of a virus related to human adenovirus. Proc. Soc. Exp. Biol. Med. 102: 1-4, 1959. PubMed: 14409689 Pereira HG, et al. A short description of the adenovirus group. Virology. 20: 613-620, 1963. PubMed: 14059828
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
Bovine adenovirus 1 Cat No.:NTCC-AVR313 Classification Adenoviridae, Mastadenovirus, Bovine mastadenovirus A Deposited As Bovine adenovirus type 1 Agent Bovine adenovirus 1 Strain 10 Applications Respiratory research Biosafety Level 2 Isolation Feces of normal calf Product Format frozen 1.0 mL Images Comments REFERENCE STRAIN: Pereira HG et al. A short description of the Adenovirus group. Virology 20: 613-620, 1963. Agglutinates rat RBC. CPE may be minimal, frequently demonstrating less than 25% involvement of cell sheet, even with prolonged incubation. AAV reported to be present. Effect on Host CPE, cell rounding with vacuolization, clumping, and sloughing Recommended Host MDBK (NBL-1) cells (ATCC® CCL-22™) Growth Conditions Temperature: 37°C Recommendations for Infection: For best results, infection should be performed on a 70-80% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 4-10 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. Effect on Host CPE, cell rounding with vacuolization, clumping, and sloughing Source Feces of normal calf References Klein M, et al. Isolation from cattle of a virus related to human adenovirus. Proc. Soc. Exp. Biol. Med. 102: 1-4, 1959. PubMed: 14409689 Pereira HG, et al. A short description of the adenovirus group. Virology. 20: 613-620, 1963. PubMed: 14059828
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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