phiX174 RFII plasmid质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

phiX174 RFII plasmid质粒载体

This is the double-stranded, nicked, circular form of φX174 DNA (relaxed form). The molecular weight of φX174 DNA is 3.50 X 106 daltons it is 5,386 base pairs in length. Greater than 90% of the molecules are RF II, the remainder are RF I (supercoiled) and linear φX174 DNA. Supplied in: 10 mM Tris-HCl (pH 8.0), 1 mM EDTA. ΦX174 RF II is prepared by relaxing (nicking) ΦX174 RF I DNA in high salt at 75°C. Greater than 90% of the molecules are in the RF II form as indicated by agarose gel electrophoresis.

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