pCR-XL-TOPO plasmid vector质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥39865
- 货 号：pCR-XL-TOPO
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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pCR-XL-TOPO plasmid vector质粒载体
pCR-XL-TOPO® allows direct selection of recombinants via disruption of the lethal E. coli gene, ccdB (Bernard et al., 1994). The vector contains the ccdB gene fused to the C-terminus of the LacZα fragment. Ligation of a long PCR product disrupts expression of the lacZα-ccdB gene fusion permitting growth of only positive recombinants upon transformation. Cells that contain non-recombinant vector are killed upon plating. Therefore, blue/white screening is not required. Lastly, by gel-purifying and visualizing the PCR product on agarose gels containing crystal violet, the damaging effects of ethidium bromide in the presence of UV light are avoided leading to higher cloning efficiencies.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
pCR-XL-TOPO® allows direct selection of recombinants via disruption of the lethal E. coli gene, ccdB (Bernard et al., 1994). The vector contains the ccdB gene fused to the C-terminus of the LacZα fragment. Ligation of a long PCR product disrupts expression of the lacZα-ccdB gene fusion permitting growth of only positive recombinants upon transformation. Cells that contain non-recombinant vector are killed upon plating. Therefore, blue/white screening is not required. Lastly, by gel-purifying and visualizing the PCR product on agarose gels containing crystal violet, the damaging effects of ethidium bromide in the presence of UV light are avoided leading to higher cloning efficiencies.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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