pIJ10257 BioVector NTCC质粒载体菌种细胞基因保藏中心

pIJ10257

This vector (originally produced and published by the Buttner group 1) can be used to introduce a gene or set of genes into Streptomyces under the control of the constitutive ermE* promoter 2. This can be useful for over expression or complementation experiments. Features Hygromycin resistance gene ermE* promoter2 ΦBT1 phage integration site Origin of transfer oriT from RK2 History Backbone vector pMS81 was modified by cloning in a 330 bp fragment containing ermE*, a ribosome binding site and a multicloning site isolated from pIJ8723, to create pIJ10257

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