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pCAP03
The pCAP03 plasmid has been successfully used to create capture vectors that can be used for TAR cloning of biosynthetic gene clusters. 'Capture arms' for a target BGC can be cloned within the restriction enzymes sites, and the plasmid contains elements that allow transfer between E. coli, actinobacteria and yeast cells. Features Apramycin resistance cassette (acc(3)IV) Neomycin and kanamycin resistance ΦC31 integrative site & integrase Origin of replication for Escherichia coli (pUC ori) ARSH4 autonomous replication sequence and CEN6 centromere for replication in yeast TRP1 marker for selection of yeast on tryptophan deficient media URA3 gene under the strong promoter pADH1 to select against non-homologous end joining in the presence of 5-fluoroorotic acid (5-FOA).
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
The pCAP03 plasmid has been successfully used to create capture vectors that can be used for TAR cloning of biosynthetic gene clusters. 'Capture arms' for a target BGC can be cloned within the restriction enzymes sites, and the plasmid contains elements that allow transfer between E. coli, actinobacteria and yeast cells. Features Apramycin resistance cassette (acc(3)IV) Neomycin and kanamycin resistance ΦC31 integrative site & integrase Origin of replication for Escherichia coli (pUC ori) ARSH4 autonomous replication sequence and CEN6 centromere for replication in yeast TRP1 marker for selection of yeast on tryptophan deficient media URA3 gene under the strong promoter pADH1 to select against non-homologous end joining in the presence of 5-fluoroorotic acid (5-FOA).
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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