pET28a-DsRed2（Kan）原核红色荧光表达质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥19865
- 货 号：pET28a-DsRed2（Kan）
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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pET28a-DsRed2（Kan）原核红色荧光表达质粒载体
pET28a-DsRed2 Plasmid InformationPromoter: T7/lacReplicator: ColE1 ori, F1 oriTerminator: T7 TerminatorPlasmid classification: Escherichia coli plasmid, large intestine fluorescent plasmid, large intestine red plasmid.Plasmid size: 6025bpPlasmid tag: N-6 * His, N-Thrombin, N-T7, N-DsRed2, C-6 * His.Prokaryotic resistance: kanamycin Kan (50 mu g/ml)Cloned strains of Escherichia coli, DH5 A and other Escherichia coliCulture conditions: 37 centigrade, aerobic, LBExpression of host: BL21 (DE3) and other Escherichia coliCulture conditions: 37 centigrade, aerobic, LBInducement: IPTG or lactose and its analogues5'sequencing primers: T7 (TAATACGACTCACTATAGGG)3'sequencing primers: T7-ter (TGCTAGTTATTGCTCAGCGG) pET28a-DsRed2 Plasmid DescriptionpET28a-DsRed2 plasmid is a prokaryotic expression vector. The C terminal contains a 6 x His label, and the N end contains a thrombin enzyme cut site, a 6 x His label, a T7 label and a DsRed2 label. The plasmid contains several commonly used restriction sites to facilitate cloning of different genes. Expression of T7 RNA polymerase provided by the host cell is induced, and the target gene is cloned into plasmid vector, which is controlled by the strong transcription and translation signal of phage.PET system is the most powerful system to express recombinant protein in Escherichia coli, and it is also the most widely used system in prokaryotic expression. The plasmid can reduce protein expression easily by decreasing the concentration of inducers. Under the condition of non inducement, the target gene can be completely silenced without transcribing.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
pET28a-DsRed2 Plasmid InformationPromoter: T7/lacReplicator: ColE1 ori, F1 oriTerminator: T7 TerminatorPlasmid classification: Escherichia coli plasmid, large intestine fluorescent plasmid, large intestine red plasmid.Plasmid size: 6025bpPlasmid tag: N-6 * His, N-Thrombin, N-T7, N-DsRed2, C-6 * His.Prokaryotic resistance: kanamycin Kan (50 mu g/ml)Cloned strains of Escherichia coli, DH5 A and other Escherichia coliCulture conditions: 37 centigrade, aerobic, LBExpression of host: BL21 (DE3) and other Escherichia coliCulture conditions: 37 centigrade, aerobic, LBInducement: IPTG or lactose and its analogues5'sequencing primers: T7 (TAATACGACTCACTATAGGG)3'sequencing primers: T7-ter (TGCTAGTTATTGCTCAGCGG) pET28a-DsRed2 Plasmid DescriptionpET28a-DsRed2 plasmid is a prokaryotic expression vector. The C terminal contains a 6 x His label, and the N end contains a thrombin enzyme cut site, a 6 x His label, a T7 label and a DsRed2 label. The plasmid contains several commonly used restriction sites to facilitate cloning of different genes. Expression of T7 RNA polymerase provided by the host cell is induced, and the target gene is cloned into plasmid vector, which is controlled by the strong transcription and translation signal of phage.PET system is the most powerful system to express recombinant protein in Escherichia coli, and it is also the most widely used system in prokaryotic expression. The plasmid can reduce protein expression easily by decreasing the concentration of inducers. Under the condition of non inducement, the target gene can be completely silenced without transcribing.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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