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轮状病毒Rotavirus A VR-2018 virus BioVector NTCC质粒载体菌种细胞基因保藏中心

  • 价  格:¥986935
  • 货  号:轮状病毒VR2018
  • 产  地:北京
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轮状病毒Rotavirus A VR-2018 virus

Classification Reoviridae, Rotavirus, Rotavirus A Deposited As Human rotavirus Agent Rotavirus A Strain Wa (TC adapted) Verified By Whole-genome Sequencing Applications Efficacy testing Testing Testing virucides Viruscide testing Enteric Research Biosafety Level 2
Comments VP7 serotype 1 (G1). Effect on Host CPE, refractile rounding and sloughing.
Recommended Host MA-104 Clone 1 (ATCC® CRL-2378.1™) Growth Conditions Temperature: 37°C Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere while continuously rocking. End adsorption by adding virus growth medium. Incubation: 1-7 days
Effect on Host CPE, refractile rounding and sloughing.
U.S. Patent 4,341,870 U.S. Patent Number 4,341,870

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