pAGEnt粪肠球菌表达载体质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心

pAGEnt粪肠球菌表达载体质粒

the exposure to the induction factor (agmatine) results in the transcription of genes under the control of the aguB promoter, including the aguBDAC operon. A novel E. faecalis expression vector, named pAGEnt, combining the aguR inducer gene and the aguB promoter followed by a cloning site and a stop codon was constructed. pAGEnt was designed for the overexpression and purification of a protein fused to a 10-amino-acid His-tag at the C-terminus. The use of GFP as a reporter of gene expression in E. faecalis revealed that under induction with 60 mM agmatine, fluorescence reached 40 arbitrary units compared to 0 in uninduced cells.
pAGEnt vector can be used for the overexpression of recombinant proteins under the induction of agmatine in E. faecalis, with a close correlation between agmatine concentration and fluorescence when GFP was used as reporter.
Genetic map of the expression vector pAGEnt and zoom-in of the expression cassette. repC and repA, replication genes; cmr, chloramphenicol resistance gene; aguR, gene encoding the regulatory protein AguR; PaguR, aguR promoter; RBS, ribosome binding site; T, transcription terminator; PaguB, aguB agmatine-inducible promoter; TGene, open reading frame for target gene of interest; His-tag; C-terminal histidine tag. Representative restriction sites are indicated.

Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net