LIM1215 cell line人结直肠癌细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥98685
- 货 号：LIM1215 cell line
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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LIM1215 cell line人结直肠癌细胞株
LIM1215 cell line Cat No.: NTCC-E10092301 Cell Line Name: LIM1215 Keywords: Human colorectal carcinoma Cell Line Description: Colon carcinoma cell line. This cell line is distributed by NTCC on behalf of CellBank Australia, Children’s Medical Research Institute, Westmead, NSW 2145, Australia. All stock supplied is manufactured and Quality Control released at source by Cell Bank Australia. Cell Bank Australia reference number: CBA-0161 Species: Human Tissue of Origin: Colon CellType: Epithelial Growth Mode: LIM1215 is loosely adherent and grows in islands which may pile up. Biosafety Information: Unless specified otherwise, we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK) All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. Subculture Routine: A centrifugation step to remove the cryoprotectant after thawing is necessary for this cell line. Recovery from thaw may take 3 days. Optimal split ratio 1:2-1:4 (seeding density 2 x104 cells/cm2).Cells incubated at 37°C with 5% CO2.Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min. Culture Medium: RPMI1640 (with 2mM L-Glutamine + 25mM HEPES) + 10% FCS, Insulin 0.6µg/ml, Hydrocortisone 1µg/ml, 1-Thioglycerol 10 µM Country: AUSTRALIA References: J Natl Cancer Inst. 1985 Apr;74(4):759-65
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
LIM1215 cell line Cat No.: NTCC-E10092301 Cell Line Name: LIM1215 Keywords: Human colorectal carcinoma Cell Line Description: Colon carcinoma cell line. This cell line is distributed by NTCC on behalf of CellBank Australia, Children’s Medical Research Institute, Westmead, NSW 2145, Australia. All stock supplied is manufactured and Quality Control released at source by Cell Bank Australia. Cell Bank Australia reference number: CBA-0161 Species: Human Tissue of Origin: Colon CellType: Epithelial Growth Mode: LIM1215 is loosely adherent and grows in islands which may pile up. Biosafety Information: Unless specified otherwise, we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK) All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. Subculture Routine: A centrifugation step to remove the cryoprotectant after thawing is necessary for this cell line. Recovery from thaw may take 3 days. Optimal split ratio 1:2-1:4 (seeding density 2 x104 cells/cm2).Cells incubated at 37°C with 5% CO2.Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min. Culture Medium: RPMI1640 (with 2mM L-Glutamine + 25mM HEPES) + 10% FCS, Insulin 0.6µg/ml, Hydrocortisone 1µg/ml, 1-Thioglycerol 10 µM Country: AUSTRALIA References: J Natl Cancer Inst. 1985 Apr;74(4):759-65
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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