LIM1863 cell line人散发性结直肠癌细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

LIM1863 cell line人散发性结直肠癌细胞株

Cell Line Name: LIM1863 Cell Line Description: LIM1863 is a cell line derived from a sporadic colorectal cancer (CRC), from a 74- year-old female patient. The cells grow as organoids and are organized around a central lumen. Both morphologically mature columnar and goblet cells are present in the organoids. LIM1863 will adhere in response to TGF-beta Species: Human (Homo sapiens) Tissue of Origin: Carcinoma of ileoceacal valve Morphology: LIM1863 grows as floating organoids Growth Mode: Suspension Biosafety Information: Unless specified otherwise,we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK) All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. Subculture Routine: Remove protective cryoflex layer around the ampoule prior to thawing. Thaw the ampoule by gently agitating in a 37°C waterbath; thawing should be rapid (around 2 minutes). A centrifugation step to remove the cryoprotectant after thawing is necessary for this cell line. Medium Renewal: 3 times per week. Subcultivation ratio: 1:4, Split once a week at this optimal ratio. To subculture, disperse the organoids into smaller clusters by vigorous pipetting and then split 1:4 to seed. Note: this cell line does not tolerate low density. If there are too few organoids in the flask to grow well they may need to be concentrated into a smaller volume. To obtain an estimated cell count, remove an aliquot of the cells suspension and after PBS wash, use 0.05% Trypsin/EDTA at 37°C for 5 minutes with mechanical dispersing before cell count. (Seeding density 2-4 x104 cells/cm2). Culture conditions: Incubate the culture at 37oC with 5% CO2. Cryoprotectant Medium: 10% DMSO + 90% FCS Culture Medium: RPMI 1640 (with 2mM L-Glutamine + 25mM HEPES) +10% FBS+ 1ug/ml Hydrocortisone + 0.6ug/ml Insulin + 10uM Thioglycerol References: Whitehead RH, Jones JK, Gabriel A, Lukies RE. A new colon carcinoma cell line (LIM1863) that grows as organoids with spontaneous differentiation into crypt-like structures in vitro. Cancer Res 1987; 47:2683-9. PMID: 3567898

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