Monster Green Fluorescent Protein phMGFP Vector plasmid高密度绿色荧光表达载体质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥19865
- 货 号：phmagfp Monster Green Fluorescent
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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Monster Green FluorescentProtein phMGFP Vector plasmid高密度绿色荧光表达载体质粒
The phMGFP Vector(a–d) contains the open reading frame for the Monster Green®Fluorescent Protein cloned into a mammalian expression vector. The MonsterGreen® Fluorescent Protein is encoded by an improved synthetic version of thegreen fluorescent protein gene originally cloned from Montastrea cavernosa (GreatStar Coral). The synthetic gene (hMGFP) expresses a 26kDa protein that showsimproved fluorescence intensity compared to the native gene. Furthermore, thehMGFP gene has been codon optimized and cleared of most consensustranscription factor binding sites to ensure reliability and high expression levels.The spectral properties of the Monster Green® Fluorescent Protein are slightlyred-shifted compared to other commercially available GFPs. Peak excitationoccurs at 505nm, with a shoulder at 480nm; peak emission occurs at 515nm. Werecommend using standard fluoroisothiocyanate (FITC) filters to visualizehMGFP fluorescence. Fluorescent microscopy analysis of hMGFP expression maybe performed using an excitation filter of 470±20nm (470/40nm) and an emissionfilter of 515nm (long pass). For FACS® analyses, we recommend using a laser at488nm for excitation and filters of 530±15nm (530/30nm) for emission.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
The phMGFP Vector(a–d) contains the open reading frame for the Monster Green®Fluorescent Protein cloned into a mammalian expression vector. The MonsterGreen® Fluorescent Protein is encoded by an improved synthetic version of thegreen fluorescent protein gene originally cloned from Montastrea cavernosa (GreatStar Coral). The synthetic gene (hMGFP) expresses a 26kDa protein that showsimproved fluorescence intensity compared to the native gene. Furthermore, thehMGFP gene has been codon optimized and cleared of most consensustranscription factor binding sites to ensure reliability and high expression levels.The spectral properties of the Monster Green® Fluorescent Protein are slightlyred-shifted compared to other commercially available GFPs. Peak excitationoccurs at 505nm, with a shoulder at 480nm; peak emission occurs at 515nm. Werecommend using standard fluoroisothiocyanate (FITC) filters to visualizehMGFP fluorescence. Fluorescent microscopy analysis of hMGFP expression maybe performed using an excitation filter of 470±20nm (470/40nm) and an emissionfilter of 515nm (long pass). For FACS® analyses, we recommend using a laser at488nm for excitation and filters of 530±15nm (530/30nm) for emission.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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