alpha TC1 clone 6 cell line|小鼠胰岛素瘤胰岛a细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
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- 货 号：alpha TC1 clone 6|小鼠胰岛素瘤胰岛a细胞
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alpha TC1 clone 6 cell line|小鼠胰岛素瘤胰岛a细胞株
alpha TC1 clone 6 CRL-2934 Organism: Mus musculus, mouse / Cell Type: alpha cells / Tissue: pancreas, alpha cells / Disease: adenomaOrganism Mus musculus, mouseTissue pancreas, alpha cellsCell Type alpha cellsProduct Format frozenMorphology epithelialCulture Properties adherent, with loosely attached clusters and some single cells in suspensionBiosafety Level 2 [Cells contain Papovavirus (SV40)]Derivation Alpha TC1 clone 6 is a pancreatic alpha cell line. It was cloned from the alpha TC1 cell line, which was derived from an adenoma created in transgenic mice expressing the SV40 large T antigen oncogene under the control of the rat preproglucagon promoter.Receptor Expression H-2b; H-2d, expressedGenes Expressed glucagonCellular Products glucagonComments The parental cell line is less differentiated and produces both glucagon and insulin. Two clonal cell lines, alpha TC1 clone 6 and alpha TC1 clone 9 (ATCC CRL-2350), are more differentiated than the parent line and produce only glucagon Ref. alpha TC1 clone 6 cells exhibit the most differentiated phenotype and express the highest levels of glucagon. RefComplete Growth Medium The base medium for this cell line is Dulbecco's Modified Eagle's Medium, low glucose (Gibco Cat. No. 11885-084). To make the complete growth medium, add the following components to the base medium:Fetal bovine serum (FBS) to a final concentration of 10%HEPES to a final concentration of 15 mMNon-essential amino acids to a final concentration of 0.1 mMBovine serum albumin to a final concentration of 0.02%Subculturing Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.NOTE: Warm all solutions to 37.0°C prior to useTransfer all medium and floating cells from flask to a 50 mL centrifuge tube.Adherent cells are removed using Cell Dissociation Buffer (an enzyme free buffer; Invitrogen, Catalog No. 13150-016) diluted 1:5 with Hanks' Balanced Salt Solution. Add 5.0 mL of diluted cell dissociation buffer per 75 cm2 flask and gently rock flask to bathe the cells at room temperature for 1 to 2 minutes.Allow the flask to remain at room temperature for 1 to 5 additional minutes until cells have detached from the flask.Firmly tap the flask against palm of hand to dislodge cells.Add 10.0 mL of fresh medium per 75 cm2 flask and triturate up and down directing the stream along the growth surface of the flask to dislodge the cells and break up some of the clumps.Transfer these cells to the centrifuge tube from Step 1. Centrifuge at 125 x g for 5 to 10 minutes. Remove medium and resuspend pellet in fresh complete medium.Add appropriate aliquots of cell suspension to new culture vessels.Incubate cultures at 37°C.Subcultivation ratio: A subcultivation ratio of 1:3 to 1:4 is recommended.Medium renewal: Every 2 to 3 days.Cryopreservation Freeze medium: complete growth medium supplemented with an additional 40% heat-inactivated fetal bovine serum and 5% (v/v) DMSOCulture Conditions Temperature: 37°CAtmosphere: air, 90%; carbon dioxide (CO2), 10%Year of Origin 1988References Powers AC, et al. Proglucagon processing similar to normal islets in pancreatic alpha-like cell line derived from transgenic mouse tumor. Diabetes 39: 406-414, 1990. PubMed: 2156740
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
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alpha TC1 clone 6 CRL-2934 Organism: Mus musculus, mouse / Cell Type: alpha cells / Tissue: pancreas, alpha cells / Disease: adenomaOrganism Mus musculus, mouseTissue pancreas, alpha cellsCell Type alpha cellsProduct Format frozenMorphology epithelialCulture Properties adherent, with loosely attached clusters and some single cells in suspensionBiosafety Level 2 [Cells contain Papovavirus (SV40)]Derivation Alpha TC1 clone 6 is a pancreatic alpha cell line. It was cloned from the alpha TC1 cell line, which was derived from an adenoma created in transgenic mice expressing the SV40 large T antigen oncogene under the control of the rat preproglucagon promoter.Receptor Expression H-2b; H-2d, expressedGenes Expressed glucagonCellular Products glucagonComments The parental cell line is less differentiated and produces both glucagon and insulin. Two clonal cell lines, alpha TC1 clone 6 and alpha TC1 clone 9 (ATCC CRL-2350), are more differentiated than the parent line and produce only glucagon Ref. alpha TC1 clone 6 cells exhibit the most differentiated phenotype and express the highest levels of glucagon. RefComplete Growth Medium The base medium for this cell line is Dulbecco's Modified Eagle's Medium, low glucose (Gibco Cat. No. 11885-084). To make the complete growth medium, add the following components to the base medium:Fetal bovine serum (FBS) to a final concentration of 10%HEPES to a final concentration of 15 mMNon-essential amino acids to a final concentration of 0.1 mMBovine serum albumin to a final concentration of 0.02%Subculturing Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.NOTE: Warm all solutions to 37.0°C prior to useTransfer all medium and floating cells from flask to a 50 mL centrifuge tube.Adherent cells are removed using Cell Dissociation Buffer (an enzyme free buffer; Invitrogen, Catalog No. 13150-016) diluted 1:5 with Hanks' Balanced Salt Solution. Add 5.0 mL of diluted cell dissociation buffer per 75 cm2 flask and gently rock flask to bathe the cells at room temperature for 1 to 2 minutes.Allow the flask to remain at room temperature for 1 to 5 additional minutes until cells have detached from the flask.Firmly tap the flask against palm of hand to dislodge cells.Add 10.0 mL of fresh medium per 75 cm2 flask and triturate up and down directing the stream along the growth surface of the flask to dislodge the cells and break up some of the clumps.Transfer these cells to the centrifuge tube from Step 1. Centrifuge at 125 x g for 5 to 10 minutes. Remove medium and resuspend pellet in fresh complete medium.Add appropriate aliquots of cell suspension to new culture vessels.Incubate cultures at 37°C.Subcultivation ratio: A subcultivation ratio of 1:3 to 1:4 is recommended.Medium renewal: Every 2 to 3 days.Cryopreservation Freeze medium: complete growth medium supplemented with an additional 40% heat-inactivated fetal bovine serum and 5% (v/v) DMSOCulture Conditions Temperature: 37°CAtmosphere: air, 90%; carbon dioxide (CO2), 10%Year of Origin 1988References Powers AC, et al. Proglucagon processing similar to normal islets in pancreatic alpha-like cell line derived from transgenic mouse tumor. Diabetes 39: 406-414, 1990. PubMed: 2156740
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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