pipkb004 plasmid vector质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pipkb004 plasmid vector质粒载体

The functionality of pIPKb002 to pIPKb005 was tested by introducing the gus gene into the GATEWAY destination
site, followed by Agrobacterium-mediated transformation of barley and the subsequent expression analysis of stable
transgenic plants. The transformation efficiency of the IPKb plasmids was on a par with that of conventional 6U-based
binary vectors without a GATEWAY cassette5
. The resultant T1 seedlings expressed GUS, with the strongest expression
present in the leaves of lines transformed with pIPKb002_GUS (driven by the ZmUbi1 promoter), followed by pIPKb003_
GUS (OsAct1 promoter), pIPKb005_GUS (TaGstA1 promoter), and pIPKb004_GUS (d35S promoter). For the transgenic
pIPKb005_GUS lines, fluorescence spectroscopy revealed that GUS activity in isolated abaxial epidermis was, on average,
ten times stronger than in remaining leaf tissue. This result not only provides an example of tissue-specific transgene
expression achieved through the use of an IPKb vector, but also opens the way to their use as a tool for studying and
manipulating the interaction between barley and many of its pathogens.
Promoter Promoter
specificity
Over-expression
vector
RNAi-vector
variable (multiple cloning site) - pIPKb001 pIPKb006
maize ubiquitin1 ubiquitous pIPKb002 pIPKb007
rice actin1 ubiquitous pIPKb003 pIPKb008
doubled enhanced CaMV35S ubiquitous pIPKb004 pIPKb009
wheat glutathion-S-transferase1 epidermis pIPKb005 pIPKb010

Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net