pCALSR1，pCALSR2-XSB植物病毒表达质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心
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pCALSR1，pCALSR2-XSB植物病毒表达质粒载体
Schematic representation of infectious clones of ALSV RNAs and infectivity of ALSV-based vector in N. benthamiana plants. (A) The infectious ALSV RNA1 and RNA2 cDNA sequences (Li et al., 2004) were respectively, introduced between the CaMV 35S RNA promoter (35S) and the nopaline synthase terminator (NOS) within the left and right borders (LB and RB) of the pCAMBIA1300 binary vector to produce pCALSR1 and pCALSR2-XSB. Restriction sites (Mlu I-Nco I [MN], Mlu I-Sal I [MS], Nco-Sal I [NS], and Sal I-Mlu I [SM]) were respectively, introduced into pALSR2-XSB immediately downstream of the translation stop codon of the 108K open reading frame to generate pCALSR2-XSB/MN, pCALSR2-XSB/MS, pCALSR2-XSB/NS, and pCALSR2-XSB/SM. The target genes can be cloned into the Xho I, Sma I, and Bam HI (XSB) restriction sites between the MP and Vp25 duplicate cleavage sites or into the MN, MS, NS, or SM restriction sites immediately downstream of the translation stop codon of the the 108K open reading frame. The open reading frames of ALSV represent the protease co-factor (Pro-co), NTP-binding helicase (HEL), cysteine protease (C-Pro), RNA polymerase (POL), movement protein (MP), and three capsid proteins (Vp25, Vp20, and Vp24). (B) N. benthamiana plants were inoculated with empty vector (Mock), pCALSR1 + pCALSR2-XSB (ALSV), and pCALSR1 + pCALSR2-XSB:NbPDS (ALSV:PDS) by agroinoculation. Infection with ALSV (pCALSR1 + pCALSR2-XSB) is symptomless and infection with ALSV:PDS (pCALSR1 + pCALSR2-XSB:NbPDS) causes photobleaching of leaves by 14 days post-inoculation.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
Schematic representation of infectious clones of ALSV RNAs and infectivity of ALSV-based vector in N. benthamiana plants. (A) The infectious ALSV RNA1 and RNA2 cDNA sequences (Li et al., 2004) were respectively, introduced between the CaMV 35S RNA promoter (35S) and the nopaline synthase terminator (NOS) within the left and right borders (LB and RB) of the pCAMBIA1300 binary vector to produce pCALSR1 and pCALSR2-XSB. Restriction sites (Mlu I-Nco I [MN], Mlu I-Sal I [MS], Nco-Sal I [NS], and Sal I-Mlu I [SM]) were respectively, introduced into pALSR2-XSB immediately downstream of the translation stop codon of the 108K open reading frame to generate pCALSR2-XSB/MN, pCALSR2-XSB/MS, pCALSR2-XSB/NS, and pCALSR2-XSB/SM. The target genes can be cloned into the Xho I, Sma I, and Bam HI (XSB) restriction sites between the MP and Vp25 duplicate cleavage sites or into the MN, MS, NS, or SM restriction sites immediately downstream of the translation stop codon of the the 108K open reading frame. The open reading frames of ALSV represent the protease co-factor (Pro-co), NTP-binding helicase (HEL), cysteine protease (C-Pro), RNA polymerase (POL), movement protein (MP), and three capsid proteins (Vp25, Vp20, and Vp24). (B) N. benthamiana plants were inoculated with empty vector (Mock), pCALSR1 + pCALSR2-XSB (ALSV), and pCALSR1 + pCALSR2-XSB:NbPDS (ALSV:PDS) by agroinoculation. Infection with ALSV (pCALSR1 + pCALSR2-XSB) is symptomless and infection with ALSV:PDS (pCALSR1 + pCALSR2-XSB:NbPDS) causes photobleaching of leaves by 14 days post-inoculation.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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