SIRT1-H363Y plasmid vector基因表达质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

SIRT1-H363Y plasmid vector基因表达质粒载体

Vector backbonepECE
Backbone size w/o insert (bp)2900
Vector typeMammalian Expression
GROWTH IN BACTERIA
Bacterial Resistance(s)Ampicillin
Growth Temperature37°C
Growth Strain(s)DH5alpha
Copy numberHigh Copy
GENE/INSERT
Gene/Insert nameSIRT1
Alt nameSir2
SpeciesH. sapiens (human)
MutationH363Y, deacetylase domain mutation
Entrez GeneSIRT1 (a.k.a. SIR2, SIR2L1, SIR2alpha)
Tag / Fusion Protein
Flag (N terminal on insert)
CLONING INFORMATION
Cloning methodRestriction Enzyme
5′ cloning siteHindIII (not destroyed)
3′ cloning siteXbaI (not destroyed)
5′ sequencing primerSV40pro-F
3′ sequencing primerSV40pA-R

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