Henle-407 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

Henle-407 cell line细胞株

Henle-407 cell line Cat No.: NTCC-ACCL6 Organism Homo sapiens, human Tissue HeLa contaminant Product Format frozen Morphology epithelial Culture Properties adherent Biosafety Level 2 [Cells contain human papilloma virus (HPV-18)] Karyotype NOTE: Cells of this line contain HeLa marker chromosomes, and were derived via HeLa contamination. HeLa Marker Chromosomes: One copy of M1, one copy of M2, four copies of M3 and one copy of M4 as revealed by G-banding patterns. Derivation This line was originally thought to be derived from normal embryonic intestinal tissue, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination. Clinical Data female HeLa Markers Y Genes Expressed keratin Cellular Products keratin Tumorigenic Yes Effects Yes, in hamster (untreated) cheek pouch Yes, in immunosuppressed rats Virus Susceptibility Human poliovirus 1 Human poliovirus 2 Human poliovirus 3 Vesicular stomatitis, Orsay (Indiana) Vesicular stomatitis, Glasgow (Indiana) Human adenovirus 3 Herpes simplex virus Pseudorabies virus Vaccinia virus Human coxsackievirus B1 Human Coxsackievirus B 2 Human Coxsackievirus B3 Human Coxsackievirus B4 Human Coxsackievirus B 5 Human Coxsackievirus B 6 Mumps rubulavirus , Mumps virus Newcastle disease virus Virus Resistance rabiesvirus Comments The cells are positive for keratin by immunoperoxidase staining Cells contain human papilloma virus (HPV-18). Complete Growth Medium Basal medium (Eagle) in Earle's BSS, 90%; fetal bovine serum, 10% Subculturing Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended Medium Renewal: Every 2 to 3 days Cryopreservation Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage Temperature: liquid nitrogen vapor phase Culture Conditions Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C STR Profile Amelogenin: X CSF1PO: 9,10 D13S317: 12,13.3 D16S539: 9,10 D5S818: 11,12 D7S820: 8,12 TH01: 7 TPOX: 8,12 vWA: 16,18 Isoenzymes G6PD, A Year of Origin January, 1955 References Henle G, Deinhardt F. The establishment of strains of human cells in tissue culture. J. Immunol. 79: 54-59, 1957. PubMed: 13475808

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