pDOE-05 plasmid植物单质粒双分子荧光互补表达载体BiFC质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心

pDOE-05 plasmid植物单质粒双分子荧光互补表达载体BiFC质粒

pDOE-05 is a single-vector self-contained BiFC construct based on the mVenus210 fragment system, and the specific fusion orientation for this construct is X::NmVenus210--X::CVenus210. The linker for the X::CVenus210 fragment contains a StrepII-FLAG-StrepII linker for detection or pull-downs. The dual ORF expresssion cassettes are driven by identical single 35S promotors with fused Omega viral translation enhancers. The pDOE-05 construct also features an independent integrated cassette that expresses the XT-Golgi-mTq2 marker from the MAS 2' promotor to specifically label the medial Golgi cisternae with mTurquoise2. Bacterial selection: Kanamycin (50 ug/mL).
Host Strain
Genus Species Strain Name
Escherichia coli TOP-10
Promoter:Reporter Names CaMV:
Promoter:Reporter Information MCS1 and MCS3 are both driven by identical single copy CaMV 35S promotors fused to an Omega 5 prime translation enhancer: mVenus210 based BiFC, mTurquoise2 Golgi marker
Selection Kanamycin (50 ug/mL)

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