pUSF-5 BAC质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pUSF-5 BAC质粒载体

Schematic representation of the strategy used to construct infectious HSV-1 BAC (a) and HSV-1 BAC Luc (b). a A BAC backbone plasmid, pUSF5 (A), contains the prokaryotic replication origin (ori) gene, the replication and partition (repE, parA, and parB) genes, the Cam r gene, and a green fluorescent protein (gfp) gene. A transfer vector, pUSF-6 (B), was constructed by inserting two 1.3-kb HSV-1 fragments (gray bars UL37 and UL38) flanked by two loxP sites (two white bars) into pUSF5. Vero cells were cotransfected with pUSF6 and the HSV-1 genome (C). Following classical homologous recombination, the BAC backbone was introduced into the intergenic region between UL37 and UL38 of the HSV-1 genome to make the

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