BTH101 E.coli strain细菌双杂交菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥98635
- 货 号：BTH101 E.coli strain细菌双杂交菌株
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
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地址：北京
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BTH101 E.coli strain细菌双杂交菌株
Two non-reverting adenylate cyclase deficient (cya) E. coli reporterstrains, BTH101 and DHM1, that are used as host organisms for detection of protein-protein interactions.The different genetic backgrounds of these two strains provide different complementation efficiencies and different reporter gene stringencies. BTH101 displays an exquisite BACTH efficiency and fast growth but some unstability of plasmids can appear due to the Rec+ character of the strain. DHM1 is a recA strain with a lower complementation efficiency and slower growth. The frequencies of spontaneous Lac+ and Mal+ revertants (because of cAMP/CAP independent promoter mutations) are respectively, 10-8 and 10-9 in BTH101 and 10-7 and 10-9 in DHM1.Strain genotype:DHM1: F-, cya-854, recA1, endA1, gyrA96 (Nal r), thi1, hsdR17, spoT1, rfbD1, glnV44(AS).BTH101: F-, cya-99, araD139, galE15, galK16, rpsL1 (Str r), hsdR2, mcrA1, mcrB1.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
Two non-reverting adenylate cyclase deficient (cya) E. coli reporterstrains, BTH101 and DHM1, that are used as host organisms for detection of protein-protein interactions.The different genetic backgrounds of these two strains provide different complementation efficiencies and different reporter gene stringencies. BTH101 displays an exquisite BACTH efficiency and fast growth but some unstability of plasmids can appear due to the Rec+ character of the strain. DHM1 is a recA strain with a lower complementation efficiency and slower growth. The frequencies of spontaneous Lac+ and Mal+ revertants (because of cAMP/CAP independent promoter mutations) are respectively, 10-8 and 10-9 in BTH101 and 10-7 and 10-9 in DHM1.Strain genotype:DHM1: F-, cya-854, recA1, endA1, gyrA96 (Nal r), thi1, hsdR17, spoT1, rfbD1, glnV44(AS).BTH101: F-, cya-99, araD139, galE15, galK16, rpsL1 (Str r), hsdR2, mcrA1, mcrB1.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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