pBBR122广宿主质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pBBR122广宿主质粒载体

pBBR122broad-host-range vector, not mobilizableThe plasmid pBBR122is a cloning vector with very broad-host-range maintenance properties. Unlikeother broad-host-range vectors, it replicates at medium copy numbers and has a small size (5307bp). This greatly facilitates genetic studies of a wide variety of Gram-negative bacteria and makes pBBR122 particularly interesting for studies of broad-host-range replicons. Being a versatile expression vector, it stably replicates in all Gram-negative organisms tried so far under standard growth conditions (seelistbelow) using appropriate selective pressure.pBBR122 carries a nonfunctional copy of the mobilization gene (mob’). Therefore it is neither mobilizable nor conjugative. For cloning and selection there are two resistance genes placed within the vector, kan (kanamycin resistance) and cm (chloramphenicol resistance). The rep gene is essential for replication. pBBR122 was derived from pBBR1, which was isolated from Bordetella bronchisepticaS87(Antoine & Locht, 1992; Renauld-Mongénie et al., 1996). It is compatible with other broad-host-range vectors, since it does not belong to thebroad-host-range incompatibility groups IncP, IncQ or IncW

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