pAtipiTrc plasmid促进类胡萝卜素原核表达质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pAtipiTrc plasmid促进类胡萝卜素原核表达质粒载体

Contains an Arabidopsis thaliana idi1 cDNA fused to a strong Trc promoter. Produces an active isopentenyl diphosphate isomerase (IDI) in E. coli.
This plasmid produces an active isopentenyl diphosphate isomerase (IPI) enzyme in E. coli, and can be used to enhance carotenoid production in E. coli containing carotenoid-producing plasmids, such as pAC-LYC and pAC-BETA and pAC-ZEAX, that lack a gene encoding an IPI. Use as a positive control when examining the activity of the products of prospective IPI-encoding genes or cDNAs in E. coli strain TOP10 (most other E. coli strains also are suitable) containing pAC-LYC or pAC-BETA. In this case, grow liquid cultures on a platform shaker at 28 degrees Celsius for 2-3 days in darkness, or grow on agar plates at room temperature for 3-7 days. An active IPI enzyme will result in E. coli colonies much darker in color.

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