PAC-BETA plasmid beta-carotene胡萝卜素原核表达质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥99865
- 货 号：PAC-BETA plasmid beta-carotene胡萝卜素原核表达质粒载体
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PAC-BETA plasmid beta-carotene胡萝卜素原核表达质粒载体
Contains crtE, crtB, crtI, and crtY carotenoid pathway genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces beta-carotene in Escherichia coli Modifications to backboneDeletion of adjacent 0.8- and 1.1 kB BamHI-BamHI fragments, and of a 1.1 kB Sall-Sal1 fragment served to remove most of the coding regions for the crtZ, crtX, and idi genes in plasmid pAC-EHER. The resulting plasmid, pAC-BETA, retains functional genes for geranylgeranyl diphosphate synthase (crtE), phytoene synthase (crtB), phytoene desaturase (crtI), and lycopene cyclase (crtY).Vector typelow copy number bacterial cloning vector For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter. Gene/Insert name crtE, crtY, crtI, crtB Species Erwinia herbicola Eho10 GenBank ID M87280.1 Promoter endogenous promoters
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
Contains crtE, crtB, crtI, and crtY carotenoid pathway genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces beta-carotene in Escherichia coli Modifications to backboneDeletion of adjacent 0.8- and 1.1 kB BamHI-BamHI fragments, and of a 1.1 kB Sall-Sal1 fragment served to remove most of the coding regions for the crtZ, crtX, and idi genes in plasmid pAC-EHER. The resulting plasmid, pAC-BETA, retains functional genes for geranylgeranyl diphosphate synthase (crtE), phytoene synthase (crtB), phytoene desaturase (crtI), and lycopene cyclase (crtY).Vector typelow copy number bacterial cloning vector For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter. Gene/Insert name crtE, crtY, crtI, crtB Species Erwinia herbicola Eho10 GenBank ID M87280.1 Promoter endogenous promoters
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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