pcDNA5/FRT/TO-Venus-Flag-Gateway (1124) plasmid vector质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pcDNA5/FRT/TO-Venus-Flag-Gateway (1124) plasmid vector质粒载体



Venus YFP-3xFlag was inserted via BstXI-XhoI sites (to create pcDNA5/FRT/TO-Venus-Flag, Addgene Plasmid# 40998).

The plasmid was then converted to a Gateway destination vector by inserting Gateway cassette C1, containing recombination sites attR1 and attR2, CmR, and ccdB, into the Afl II site (blunt ended).

This plasmid can be used to place genes into the pcDNA5/FRT/TO-Venus-Flag construct by Gateway reaction, to make tetracycline-inducible stable cell lines.

For subcloning, the following restriction sites are present:
Between the CMV promoter and the Gateway cassette: PmeI (non-unique)
Between the Gateway cassette and Venus: HindIII, Asp718I, KpnI, BamHI (non-unique, also in GW casette), and BstXI
Between Venus and BGHpA: XhoI, Eco0109I, ApaI, and PmeI (non-unique)


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