PG-13 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥49865
- 货 号：PG-13 cell line细胞株
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
- 已注册
PG-13 cell line细胞株
Cell Line Name: PG13Other Collection No.: ATCC CRL 10686Citation Guidance: If use of this culture results in a scientific publication, it should be cited in the publication as: PG13 (ECACC 95110215)Keywords: Mouse embryo fibroblast, GALV-based retrovirusCell Line Description: PG13 cells were derived from TK-NIH/3T3 cells. This retrovirus packaging cell line is based on the gibbon ape Leukaemia Virus (GaLV). Introduction of retroviral vectors results in the production of retrovirus virions capable of infecting cells from many species excluding mice. Introduced DNA may be lost after long-term passage. Selection against loss can be performed by growing the cells in medium with dialysed FBS and100nM amethopterin for 5 days followed by cultivation in HAT and untreated FBS for 5 days. Maintain cells for 4 days in HT medium to avoid toxic effects of residual amethopterin. Virus production has not been observed, but there is the risk that cells produce a virus similar to GaLV. The cell line has been tested positive for Reverse Transcriptase.Species: MouseTissue of Origin: EmbryoCellType: FibroblastGrowth Mode: AdherentKaryotype: Not specifiedGMO Status: Genetically Modified Organism Class 1 (GMO1)Biosafety Information: Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.Hyperlinks to MSDS documents:Frozen cell cultures Material Safety Data SheetGrowing cell cultures Material Safety Data SheetNucleic acids derived from cell cultures Material Safety Data SheetSubculture Routine: Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Culture Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Depositor: Obtained from ATCCOriginator: NoCountry: USAReferences: J Virol 1991;65:2220; Biotechniques 1989;7:980
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
Cell Line Name: PG13Other Collection No.: ATCC CRL 10686Citation Guidance: If use of this culture results in a scientific publication, it should be cited in the publication as: PG13 (ECACC 95110215)Keywords: Mouse embryo fibroblast, GALV-based retrovirusCell Line Description: PG13 cells were derived from TK-NIH/3T3 cells. This retrovirus packaging cell line is based on the gibbon ape Leukaemia Virus (GaLV). Introduction of retroviral vectors results in the production of retrovirus virions capable of infecting cells from many species excluding mice. Introduced DNA may be lost after long-term passage. Selection against loss can be performed by growing the cells in medium with dialysed FBS and100nM amethopterin for 5 days followed by cultivation in HAT and untreated FBS for 5 days. Maintain cells for 4 days in HT medium to avoid toxic effects of residual amethopterin. Virus production has not been observed, but there is the risk that cells produce a virus similar to GaLV. The cell line has been tested positive for Reverse Transcriptase.Species: MouseTissue of Origin: EmbryoCellType: FibroblastGrowth Mode: AdherentKaryotype: Not specifiedGMO Status: Genetically Modified Organism Class 1 (GMO1)Biosafety Information: Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.Hyperlinks to MSDS documents:Frozen cell cultures Material Safety Data SheetGrowing cell cultures Material Safety Data SheetNucleic acids derived from cell cultures Material Safety Data SheetSubculture Routine: Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.Culture Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).Depositor: Obtained from ATCCOriginator: NoCountry: USAReferences: J Virol 1991;65:2220; Biotechniques 1989;7:980
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
您正在向 biovector.net 发送关于产品 PG-13 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心 的询问
- 公告/新闻
