Neuro-2a cell line小鼠神经母细胞瘤细胞系 BioVector NTCC质粒载体菌种细胞基因保藏中心
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- 货 号：Neuro-2a cell line小鼠神经母细胞瘤细胞系
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Neuro-2a cell line小鼠神经母细胞瘤细胞系
Organism Mus musculus, mouseTissue brainCell Type neuroblastProduct Format frozenMorphology neuronal and amoeboid stem cellsCulture Properties adherentBiosafety Level 1Disease neuroblastomaStrain AApplications This cell line is a suitable transfection host.The cell line has been used for studies on the mechanism of vinblastine precipitation of microtubular protein, the kinetics of GTP binding to isolated protein, the turnover of microtubules in vivo, and the synthesis and assembly of microtubular protein . The World Organization for Animal Health (OIE) uses the cells for routine diagnosis of rabies. Karyotype modal number = 95; range = 59 to 193. Karyotype unstable within a stemline range of 94 to 98 chromosomes. All the cells contain 6 to 10 large chromosomes with median or submedian centromeres and 2 to 4 minute chromosomes. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines.Images CCL-131 MicrographDerivation Clone Neuro-2a was established by R.J. Klebe and F.H. Ruddle from a spontaneous tumor of a strain A albino mouse. This tumor line, designated C1300, was obtained from the Jackson Laboratory, Bar Harbor, MaineAntigen Expression H-2, a haplotype; Mus musculus, expressedGenes Expressed acetylcholinesterase, tubulinCellular Products acetylcholinesterasetubulinVirus Susceptibility Herpes simplex virusVesicular stomatitis virusHuman poliovirus 1Comments Neuro-2a cells produce large quantities of microtubular protein which is believed to play a role in a contractile system which is responsible for axoplasmic flow in nerve cells. Tested and found negative for ectromelia virus (mousepox).Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.Subculturing Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommendedMedium Renewal: 1 to 2 times per weekCryopreservation Freeze medium: Complete growth medium, 95%; DMSO, 5%Storage temperature: liquid nitrogen vapor phaseCulture Conditions Atmosphere: air, 95%; carbon dioxide (CO2), 5%Temperature: 37°CReferences Olmsted JB, et al. Isolation of microtubule protein from cultured mouse neuroblastoma cells. Proc. Natl. Acad. Sci. USA 65: 129-136, 1970. PubMed: 5263744
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
Organism Mus musculus, mouseTissue brainCell Type neuroblastProduct Format frozenMorphology neuronal and amoeboid stem cellsCulture Properties adherentBiosafety Level 1Disease neuroblastomaStrain AApplications This cell line is a suitable transfection host.The cell line has been used for studies on the mechanism of vinblastine precipitation of microtubular protein, the kinetics of GTP binding to isolated protein, the turnover of microtubules in vivo, and the synthesis and assembly of microtubular protein . The World Organization for Animal Health (OIE) uses the cells for routine diagnosis of rabies. Karyotype modal number = 95; range = 59 to 193. Karyotype unstable within a stemline range of 94 to 98 chromosomes. All the cells contain 6 to 10 large chromosomes with median or submedian centromeres and 2 to 4 minute chromosomes. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines.Images CCL-131 MicrographDerivation Clone Neuro-2a was established by R.J. Klebe and F.H. Ruddle from a spontaneous tumor of a strain A albino mouse. This tumor line, designated C1300, was obtained from the Jackson Laboratory, Bar Harbor, MaineAntigen Expression H-2, a haplotype; Mus musculus, expressedGenes Expressed acetylcholinesterase, tubulinCellular Products acetylcholinesterasetubulinVirus Susceptibility Herpes simplex virusVesicular stomatitis virusHuman poliovirus 1Comments Neuro-2a cells produce large quantities of microtubular protein which is believed to play a role in a contractile system which is responsible for axoplasmic flow in nerve cells. Tested and found negative for ectromelia virus (mousepox).Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.Subculturing Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product. Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommendedMedium Renewal: 1 to 2 times per weekCryopreservation Freeze medium: Complete growth medium, 95%; DMSO, 5%Storage temperature: liquid nitrogen vapor phaseCulture Conditions Atmosphere: air, 95%; carbon dioxide (CO2), 5%Temperature: 37°CReferences Olmsted JB, et al. Isolation of microtubule protein from cultured mouse neuroblastoma cells. Proc. Natl. Acad. Sci. USA 65: 129-136, 1970. PubMed: 5263744
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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