Baculovirus AcMNPV Expressing E. coli Cell Line (DE35) BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥98635
- 货 号：Baculovirus AcMNPV Expressing E. coli Cell Line (DE35)
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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Baculovirus AcMNPV Expressing E. coli Cell Line (DE35)
E. coli DE35 cells with the complete Autographa californica nucleopolyhedrovirus (AcMNPV) baculovirus genome which has been modified to produce enzymes, required for high yield farnesylation in insect cells.Highlights: Cells utilizes the Tn7-based transposition strategy for production of baculovirus genomes in E. coli Contains deletions of the viral cathepsin and chitinase genes to help improve protein yield and quality The AcMNPV genome is carried on a single-copy F' origin plasmid which can be replicated in E. coli Includes protein farnesyltransferase/geranylgeranyltransferase type-1 subunit alpha (FNTA) and protein farnesyltransferase subunit beta (FNTB) See also: KRAS4b Baculovirus Expression VectorsDE35 is an analogous strain to E. coli DH10Bac cells, which utilizes the Tn7-based transposition strategy for production of baculovirus genomes in E. coli. The modified AcMNPV genome is carried on a single-copy F’ origin plasmid which can be replicated in E. coli and a subsequent alkaline lysis DNA preparation can be used to transfect insect cells to produce competent baculovirus. In addition to the introduction of human prenylation-related enzymes FNTA and FNTB , the viral genome in DE35 also includes deletions of the viral cathepsin and chitinase genes to improve protein yield and quality.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
E. coli DE35 cells with the complete Autographa californica nucleopolyhedrovirus (AcMNPV) baculovirus genome which has been modified to produce enzymes, required for high yield farnesylation in insect cells.Highlights: Cells utilizes the Tn7-based transposition strategy for production of baculovirus genomes in E. coli Contains deletions of the viral cathepsin and chitinase genes to help improve protein yield and quality The AcMNPV genome is carried on a single-copy F' origin plasmid which can be replicated in E. coli Includes protein farnesyltransferase/geranylgeranyltransferase type-1 subunit alpha (FNTA) and protein farnesyltransferase subunit beta (FNTB) See also: KRAS4b Baculovirus Expression VectorsDE35 is an analogous strain to E. coli DH10Bac cells, which utilizes the Tn7-based transposition strategy for production of baculovirus genomes in E. coli. The modified AcMNPV genome is carried on a single-copy F’ origin plasmid which can be replicated in E. coli and a subsequent alkaline lysis DNA preparation can be used to transfect insect cells to produce competent baculovirus. In addition to the introduction of human prenylation-related enzymes FNTA and FNTB , the viral genome in DE35 also includes deletions of the viral cathepsin and chitinase genes to improve protein yield and quality.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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