MOLM-13 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

MOLM-13 cell line细胞株


Cell line:
MOLM-13
DSMZ no.:
ACC 554
Species:
human (Homo sapiens)
Cell type:
acute myeloid leukemia
Origin:
established from the peripheral blood of a 20-year-old man with acute myeloid leukemia AML FAB M5a at relapse in 1995 after initial myelodysplastic syndromes (MDS, refractory anemia with excess of blasts, RAEB); carries internal tandem duplication of FLT3; the FLT3 protein is not expressed; cell line carries the CBL deltaExon8 mutant; sister cell line of MOLM-14 (ACC 777). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq)
Reference(s):
14869, 14743, 14493, 15073, 16558, 18138, 18187
Biosafety level:
1
Permissions and restrictions:
A

DSMZ Cell Culture Data:
Morphology:
most cells are round growing in suspension ; image ; image
Medium:
80-90% RPMI 1640 + 10-20% h.i. FBS
Subculture:
seed out at ca. 1.0 x 106 cells/ml; split saturated culture 1:2 to 1:3 every 2-3 days; maintain at ca 0.4-2.0 x 106 cells/ml; viability may drop below 50% after thawing, but cells recover after 4-5 days (starting in a 12- or 24-well plate is recommended)
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50 hours
Harvest:
cell harvest of ca. 2.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD4 +, CD13 (+), CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, cyCD68 +, HLA-DR -; image
Fingerprint:
multiplex PCR of minisatellite markers revealed a DNA profile identical to MOLM-14
Species:
confirmed as human by PCR speciation and cytogenetics
Cytogenetics:
human hyperdiploid karyotype with 4% polyploidy - 51(48-52)<2n>XY, +8, +8, +8, +13, del(8)(p1?p2?), ins(11;9)(q23;p22p23) - resembles published karyotype - sideline with idem, +19 - carries occult insertion effecting KMT2A-MLLT3 (MLL-MLLT3; MLL-AF9) fusion
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -


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