BB7.2 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥49865
- 货 号：BB7.2 cell line细胞株
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
- 已注册
BB7.2 cell line细胞株
BB7.2 cellsOrganism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)Cell Type hybridoma: B lymphocyteProduct Format frozenMorphology lymphoblastCulture Properties suspensionBiosafety Level 1 Derivation Spleen cells were fused with NS-1 myeloma cells.Genes Expressed immunoglobulin; monoclonal antibody; against HLA A2, Aw69; cytotoxicCellular Products immunoglobulin; monoclonal antibody; against HLA A2, Aw69; cytotoxicComments Animals were immunized with papain solubilized HLA A2 antigen.Spleen cells were fused with NS-1 myeloma cells.Mutation at position 107 of the alpha 2 domain of the HLA-A2 molecule results in partial loss of BB7.2 binding.BB7.2 and PA2.1 (ATCC HB-117) recognize overlapping epitopes on the alpha 2 domain of HLA-A2.The antibody cross-reacts with cells from various primate species.Tested and found negative for ectromelia virus (mousepox).Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.Subculturing Medium Renewal: Every 2 to 3 daysCultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.Isotype IgG2b References Parham P, Brodsky FM. Partial purification and some properties of BB7.2. A cytotoxic monoclonal antibody with specificity for HLA-A2 and a variant of HLA-A28. Hum. Immunol. 3: 277-299, 1981. PubMed: 7035415
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
BB7.2 cellsOrganism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)Cell Type hybridoma: B lymphocyteProduct Format frozenMorphology lymphoblastCulture Properties suspensionBiosafety Level 1 Derivation Spleen cells were fused with NS-1 myeloma cells.Genes Expressed immunoglobulin; monoclonal antibody; against HLA A2, Aw69; cytotoxicCellular Products immunoglobulin; monoclonal antibody; against HLA A2, Aw69; cytotoxicComments Animals were immunized with papain solubilized HLA A2 antigen.Spleen cells were fused with NS-1 myeloma cells.Mutation at position 107 of the alpha 2 domain of the HLA-A2 molecule results in partial loss of BB7.2 binding.BB7.2 and PA2.1 (ATCC HB-117) recognize overlapping epitopes on the alpha 2 domain of HLA-A2.The antibody cross-reacts with cells from various primate species.Tested and found negative for ectromelia virus (mousepox).Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.Subculturing Medium Renewal: Every 2 to 3 daysCultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.Isotype IgG2b References Parham P, Brodsky FM. Partial purification and some properties of BB7.2. A cytotoxic monoclonal antibody with specificity for HLA-A2 and a variant of HLA-A28. Hum. Immunol. 3: 277-299, 1981. PubMed: 7035415
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
您正在向 biovector.net 发送关于产品 BB7.2 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心 的询问
- 公告/新闻
