TeloHAEC cells细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥49865
- 货 号：TeloHAEC cells细胞株
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
- 已注册
TeloHAEC cells细胞株
TeloHAEC cell lineCat No.: NTCC-CRL4052Organism Homo sapiens, human Tissue aorta Cell Type endothelial Product Format frozen Morphology endothelial Culture Properties adherent Biosafety Level 2 [Cells containing SV40 viral DNA sequences]Disease normal Age 34 years old Gender female Applications TeloHAEC (NTCC CRL-4052) cells retain important endothelial cell characteristics such as CD31/PECAM-1 marker expression and LDL functional uptake; the cells also show effective inflammatory response upon TNFα treatment and increase proliferation upon VEGF stimulation. When co-cultured with fibroblasts, TeloHAEC cells can also form neoangiogenic tubular networks in vitro, which are responsive to VEGF stimulation and suramin inhibition. Karyotype This is a diploid cell line of female origin with a consistent normal kayrotype at low and high passages Images Derivation TeloHAEC is a clonal cell line immortalized by stably expressing human telomerase catalytic subunit hTERT Clinical Data female 34Antigen Expression Positive for CD31/PECAM-1 expression and capable of uptaking Low Density Lipoprotein (LDL). Complete Growth Medium The base medium for this cell line is Vascular Cell Basal Medium (NTCC PCS-100-030), supplemented with Vascular Endothelial Cell Growth Kit-VEGF (NTCC PCS-100-041). Optional: Add 0.3ug/mL Puromycin(Santa Cruz Biotech: sc-108071A). Note: Do not filter complete medium. Subculturing Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation solutions for culture vessels of other sizes.Subculture when the culture is about 90% confluent.1.Remove and discard spent medium.2.Briefly rinse the cells with Dulbecco's Phosphate Buffered Saline (DPBS, NTCC 30-2200) and discard rinse solution.3.Add 2.0 to 3.0 mL room temperature Trypsin-EDTA for Primary Cells (NTCC PCS-999-003) to the flask. Incubate at 37°C for 5 min (until cells have detached).4.Neutralize trypsin by adding an equal volume of room temperature 2% FBS in DPBS.5.Centrifuge cells at 250 x g for 5 min at room temperature.6.Remove supernatant. Resuspend pellet in 6.0 to 8.0 mL Complete Growth Medium.7.Count cells, and seed 5.0 x 103 to 8.0 x 103 viable cells/cm2 to new culture vessels.Medium Renewal: Every 2-3 days. Cryopreservation 90% FBS, 10% DMSO Culture Conditions Atmosphere: air, 95%; carbon dioxide (CO2), 5%Temperature: 37°C STR Profile D5S818: 12 D13S317: 9, 12D7S820: 10, 11 D16S539: 12, 13vWA: 15, 16 Amelogenin: XTPOX: 8CSF1PO: 11, 12TH01: 6, 8 Population Doubling Level (PDL) As part of our quality control, we have tested this cell line for its ability to grow for a minimum of 15 population doublings after recovery from cryopreservation. We have also compared its karyotype, telomerase expression level, growth rate, morphology and tissue-specific markers when first recovered from cryopreservation with that of cells at 10+ population doublings to ensure that there is no change in these parameters and that the cells are capable of extended proliferation.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
TeloHAEC cell lineCat No.: NTCC-CRL4052Organism Homo sapiens, human Tissue aorta Cell Type endothelial Product Format frozen Morphology endothelial Culture Properties adherent Biosafety Level 2 [Cells containing SV40 viral DNA sequences]Disease normal Age 34 years old Gender female Applications TeloHAEC (NTCC CRL-4052) cells retain important endothelial cell characteristics such as CD31/PECAM-1 marker expression and LDL functional uptake; the cells also show effective inflammatory response upon TNFα treatment and increase proliferation upon VEGF stimulation. When co-cultured with fibroblasts, TeloHAEC cells can also form neoangiogenic tubular networks in vitro, which are responsive to VEGF stimulation and suramin inhibition. Karyotype This is a diploid cell line of female origin with a consistent normal kayrotype at low and high passages Images Derivation TeloHAEC is a clonal cell line immortalized by stably expressing human telomerase catalytic subunit hTERT Clinical Data female 34Antigen Expression Positive for CD31/PECAM-1 expression and capable of uptaking Low Density Lipoprotein (LDL). Complete Growth Medium The base medium for this cell line is Vascular Cell Basal Medium (NTCC PCS-100-030), supplemented with Vascular Endothelial Cell Growth Kit-VEGF (NTCC PCS-100-041). Optional: Add 0.3ug/mL Puromycin(Santa Cruz Biotech: sc-108071A). Note: Do not filter complete medium. Subculturing Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation solutions for culture vessels of other sizes.Subculture when the culture is about 90% confluent.1.Remove and discard spent medium.2.Briefly rinse the cells with Dulbecco's Phosphate Buffered Saline (DPBS, NTCC 30-2200) and discard rinse solution.3.Add 2.0 to 3.0 mL room temperature Trypsin-EDTA for Primary Cells (NTCC PCS-999-003) to the flask. Incubate at 37°C for 5 min (until cells have detached).4.Neutralize trypsin by adding an equal volume of room temperature 2% FBS in DPBS.5.Centrifuge cells at 250 x g for 5 min at room temperature.6.Remove supernatant. Resuspend pellet in 6.0 to 8.0 mL Complete Growth Medium.7.Count cells, and seed 5.0 x 103 to 8.0 x 103 viable cells/cm2 to new culture vessels.Medium Renewal: Every 2-3 days. Cryopreservation 90% FBS, 10% DMSO Culture Conditions Atmosphere: air, 95%; carbon dioxide (CO2), 5%Temperature: 37°C STR Profile D5S818: 12 D13S317: 9, 12D7S820: 10, 11 D16S539: 12, 13vWA: 15, 16 Amelogenin: XTPOX: 8CSF1PO: 11, 12TH01: 6, 8 Population Doubling Level (PDL) As part of our quality control, we have tested this cell line for its ability to grow for a minimum of 15 population doublings after recovery from cryopreservation. We have also compared its karyotype, telomerase expression level, growth rate, morphology and tissue-specific markers when first recovered from cryopreservation with that of cells at 10+ population doublings to ensure that there is no change in these parameters and that the cells are capable of extended proliferation.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
您正在向 biovector.net 发送关于产品 TeloHAEC cells细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心 的询问
- 公告/新闻
