pGXHNL100 plasmid vector质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pGXHNL100 plasmid vector质粒载体

S. fredii HN01 Wild type, Nm r Amp r Preservation
S. meliloti 1021 Wild type, Sm r Amp r Preservation
E. coli DH5α SupE44△lacU169 (ψ80lacZ△M15) hsdR17 RecA1 endA1 gyrA96 thi-1 relA1 Hanahan D.[8]
GXHNLTA Positive mutants of HN01, Nmr
Kmr This work
GXHNLTB Negative mutants of HN01，Nmr
Kmr This work
GXHNWA GXHNTA containing pGXHNL100, Nmr
Tcr
Kmr This work
GXHNWB GXHNTB containing pGXHNL100, Nmr
Tcr
Kmr This work
质粒 Plasmids
pRK2013 Tra+
, Mob+
, ColE1-replicon, Km r Preservation
pGEM-T Easy Ampr Promega
pGEM-3zf(+) Ampr From Promega
pLARF3 Mob-
, Tra+
, Cosmid, Tcr Preservation
pK18mob Kmr Preservation
pK18-plrp plrp, PCR of product of lrp, positively ligated in SmalI site of pk18mob, Kmr This work
pGXHNL100 EcoRI site of pLAFR3 ligated fragment containing lrp gene, Tcr This work

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