pBluescript II KS(-)噬菌粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥19865
- 货 号：pBluescript II KS(-)噬菌粒载体
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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pBluescript II KS(-)噬菌粒载体
The pBluescript II phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and gene mapping. The pBluescript II phagemids have an extensive polylinker with 21 unique restriction enzyme recognition sites. Flanking the polylinker are T7 and T3 RNA polymerase promoters that can be used to synthesize RNA in vitro.1, 2 The choice of promoter used to initiate transcription determines which strand of the insert cloned into the polylinker will be transcribed. Circular maps and lists of features for the pBluescript II phagemids are shown in figures 1 and 2. The polylinker and T7 and T3 RNA polymerase promoter sequences are present in the N-terminal portion of a lacZ gene fragment. A total of 131 amino acids of β-galactosidase coding sequence is present in the pBluescript II phagemid, but the coding sequence is interrupted by the large polylinker. (There are 36 amino acids from the initiator Met sequence to the EcoR I site.) pBluescript II phagemids having no inserts in the polylinker will produce blue colonies in the appropriate strains of bacteria (i.e., strains containing lacZΔM15 on an F´ episome, such as XL1-Blue MRF´, among others). pBluescript II phagemids that have inserts will produce white colonies using the same strain, because the inserts disrupt the coding region of the lacZ gene fragment. pBluescript II (+) and (–) are available with two polylinker orientations designated as either KS or SK using the following convention: (1) in the KS orientation, the Kpn I restriction site is nearest the lacZ promoter and the Sac I restriction site is farthest from the lacZ promoter; and (2) in the SK orientation, the Sac I site is the closest restriction site to the lacZ promoter and the Kpn I site is the farthest. Flanking the T3 and T7 promoters are BssH II sites. This rare six-base cutter will allow the insert plus the T phage RNA promoters to be excised and used for gene mapping. pBluescript II phagemids can be rescued as single-stranded (ss) DNA. pBluescript II phagemids contain a 454-bp filamentous f1 phage intergenic region (M13 related), which includes the 307-bp origin of replication. The (+) and (–) orientations of the f1 intergenic region allow the rescue of sense or antisense ssDNA by a helper phage. This ssDNA can be used for dideoxynucleotide sequencing (Sanger method) or site-specific mutagenesis.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
The pBluescript II phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and gene mapping. The pBluescript II phagemids have an extensive polylinker with 21 unique restriction enzyme recognition sites. Flanking the polylinker are T7 and T3 RNA polymerase promoters that can be used to synthesize RNA in vitro.1, 2 The choice of promoter used to initiate transcription determines which strand of the insert cloned into the polylinker will be transcribed. Circular maps and lists of features for the pBluescript II phagemids are shown in figures 1 and 2. The polylinker and T7 and T3 RNA polymerase promoter sequences are present in the N-terminal portion of a lacZ gene fragment. A total of 131 amino acids of β-galactosidase coding sequence is present in the pBluescript II phagemid, but the coding sequence is interrupted by the large polylinker. (There are 36 amino acids from the initiator Met sequence to the EcoR I site.) pBluescript II phagemids having no inserts in the polylinker will produce blue colonies in the appropriate strains of bacteria (i.e., strains containing lacZΔM15 on an F´ episome, such as XL1-Blue MRF´, among others). pBluescript II phagemids that have inserts will produce white colonies using the same strain, because the inserts disrupt the coding region of the lacZ gene fragment. pBluescript II (+) and (–) are available with two polylinker orientations designated as either KS or SK using the following convention: (1) in the KS orientation, the Kpn I restriction site is nearest the lacZ promoter and the Sac I restriction site is farthest from the lacZ promoter; and (2) in the SK orientation, the Sac I site is the closest restriction site to the lacZ promoter and the Kpn I site is the farthest. Flanking the T3 and T7 promoters are BssH II sites. This rare six-base cutter will allow the insert plus the T phage RNA promoters to be excised and used for gene mapping. pBluescript II phagemids can be rescued as single-stranded (ss) DNA. pBluescript II phagemids contain a 454-bp filamentous f1 phage intergenic region (M13 related), which includes the 307-bp origin of replication. The (+) and (–) orientations of the f1 intergenic region allow the rescue of sense or antisense ssDNA by a helper phage. This ssDNA can be used for dideoxynucleotide sequencing (Sanger method) or site-specific mutagenesis.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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