pWPT-nlsLacZ慢病毒表达质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心

pWPT-nlsLacZ慢病毒表达质粒

pWPT or pWPXL can be used for constitutive transgene expression.
pWPXL contains the EF-1alpha promoter + intron that gives you high expression, as RNA loves to be spliced (it goes more efficiently out of the nucleus). pWPT contains only the EF-1alpha promoter

The loxP site in the 3'LTR is duplicated to the 5'LTR during reverse transcription in the target cells. This allows for removal (if necessary) of an integrated provirus by Cre.

Unique restriction sites at key positions will allow you to change the promoter and transgene.

Please note that ClaI in these vectors is blocked by Dam methylation.
The plasmids need to be grown in a Dam- bacteria strain, if you wish to use ClaI for cloning.

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