LL/2 (LLC1) cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥39865
- 货 号：LL/2 (LLC1) cell line细胞株
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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LL/2 (LLC1) cell line细胞株
Organism Mus musculus, mouseTissue lungProduct Format frozenMorphology rounded - loosely attached or floatingCulture Properties mixed, adherent and suspensionBiosafety Level 1Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.Disease Lewis lung carcinomaStrain C57BLApplications This line is widely used as a model for metastasis and is useful for studying the mechanisms of cancer chemotherapeutic agents.Derivation Lewis lung carcinoma is a cell line established from the lung of a C57BL mouse bearing a tumor resulting from an implantation of primary Lewis lung carcinoma.Antigen Expression H-2bTumorigenic YesEffects Yes, in C57BL miceComments The cells are resistant to 1,3-bis-(2-chloroethyl)-1-nitrosourea, but are sensitive to methotrexate.The cells are reported to be highly tumorigenic, but weakly metastatic in mice.The cells form multilayers in flasks without actually becoming confluent.Tested and found negative for ectromelia virus (mousepox).Complete Growth Medium The base medium for this cell line is Dulbecco's Modified Eagle's Medium, . To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.Subculturing Subcultures are prepared by diluting the suspension 1:4 to 1:6. Cells on the floor of the flask may be dislodged by aspirating several times with culture medium or by rinsing with 0.25% trypsin - 0.53 mM EDTA solution. T-75 flasks are recommended for subculturing this product.Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommendedMedium Renewal: 2 to 3 times per weekCryopreservation Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSOStorage temperature: liquid nitrogen vapor phaseCulture Conditions Temperature: 37°C
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
Organism Mus musculus, mouseTissue lungProduct Format frozenMorphology rounded - loosely attached or floatingCulture Properties mixed, adherent and suspensionBiosafety Level 1Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.Disease Lewis lung carcinomaStrain C57BLApplications This line is widely used as a model for metastasis and is useful for studying the mechanisms of cancer chemotherapeutic agents.Derivation Lewis lung carcinoma is a cell line established from the lung of a C57BL mouse bearing a tumor resulting from an implantation of primary Lewis lung carcinoma.Antigen Expression H-2bTumorigenic YesEffects Yes, in C57BL miceComments The cells are resistant to 1,3-bis-(2-chloroethyl)-1-nitrosourea, but are sensitive to methotrexate.The cells are reported to be highly tumorigenic, but weakly metastatic in mice.The cells form multilayers in flasks without actually becoming confluent.Tested and found negative for ectromelia virus (mousepox).Complete Growth Medium The base medium for this cell line is Dulbecco's Modified Eagle's Medium, . To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.Subculturing Subcultures are prepared by diluting the suspension 1:4 to 1:6. Cells on the floor of the flask may be dislodged by aspirating several times with culture medium or by rinsing with 0.25% trypsin - 0.53 mM EDTA solution. T-75 flasks are recommended for subculturing this product.Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommendedMedium Renewal: 2 to 3 times per weekCryopreservation Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSOStorage temperature: liquid nitrogen vapor phaseCulture Conditions Temperature: 37°C
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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