LLC-IL-1β cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥98635
- 货 号：LLC-IL-1β cell line细胞株
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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LLC-IL-1β cell line细胞株
EL-4 and EG-7 thymomas, B16-F10 melanoma, and LLC were obtained from ATCC. Cells were cultured in DMEM (BioSource International) supplemented with 10% FBS, 5 mM glutamine, 25 mM HEPES, and 1% antibiotics (Mediatech). The LLC-IL-1β cell line was created by transfection of pLXSN/ssIL-1β plasmid, generated by R. Apte (Ben-Gurion University of the Negev, Beer-Sheva, Israel) and provided by S. Ostrand-Rosenberg (University of Maryland, College Park, Maryland, USA) using a Nucleofector kit (Amaxa, Lonza). Selection was performed using G418 (Calbiochem). Single-cell clones were evaluated for IL-1β production by testing with an hIL-1β ELISA kit (eBioscience).
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
EL-4 and EG-7 thymomas, B16-F10 melanoma, and LLC were obtained from ATCC. Cells were cultured in DMEM (BioSource International) supplemented with 10% FBS, 5 mM glutamine, 25 mM HEPES, and 1% antibiotics (Mediatech). The LLC-IL-1β cell line was created by transfection of pLXSN/ssIL-1β plasmid, generated by R. Apte (Ben-Gurion University of the Negev, Beer-Sheva, Israel) and provided by S. Ostrand-Rosenberg (University of Maryland, College Park, Maryland, USA) using a Nucleofector kit (Amaxa, Lonza). Selection was performed using G418 (Calbiochem). Single-cell clones were evaluated for IL-1β production by testing with an hIL-1β ELISA kit (eBioscience).
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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