PBCAG-eGFP plasmid vector质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

PBCAG-eGFP plasmid vector质粒载体

pCAGGFP
(Search Vector Database)
Backbone size w/o insert (bp) 5551
Total vector size (bp) 6336
Vector type
Mammalian Expression

Growth in Bacteria

Bacterial Resistance(s)
Ampicillin
Growth Temperature
37°C
Growth Strain(s)
DH5alpha
Copy number
High Copy

Gene/Insert 1

Gene/Insert name
PiggyBac transposon 3' terminal repeats
Species
Synthetic
Insert Size (bp)
430

Cloning Information for Gene/Insert 1

Cloning method Restriction Enzyme
5′ cloning site SalI (not destroyed)
3′ cloning site SpeI (not destroyed)
5′ sequencing primer GTTATTGTCTCATGAGCGGATAC
3′ sequencing primer No
(Common Sequencing Primers)

Gene/Insert 2

Gene/Insert name
PiggyBac 5' terminal repeats
Species
Synthetic
Insert Size (bp)
369

Cloning Information for Gene/Insert 2

Cloning method Restriction Enzyme
5′ cloning site PstI (not destroyed)
3′ cloning site HindIII (not destroyed)
5′ sequencing primer No
3′ sequencing primer CCCCCTGCTGTCCATTCCTTATTC

Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net