PNT1A cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
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- 货 号：PNT1A cell line细胞株
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PNT1A cell line细胞株
Cell Line Name: PNT1A Keywords: Human post pubertal prostate normal, immortalised with SV40 Cell Line Description: Established by immortalisation of normal adult prostatic epithelial cells by transfection with a plasmid containing SV40 genome with a defective replication origin. The primary culture was obtained from the prostate of a 35 year old male at post mortem. The cells contain the SV40 genome and express large T protein. They present the phenotype of differentiated luminal prostatic cells with the expression of cytokeratin 8 and 18 (markers for luminal glandular epithelia) and vimentin. Cytokeratin14, a marker of epithelial basal cells, is not expressed. PNT1A cells are non-tumourigenic in nude mice. Species: Human Tissue of Origin: prostate CellType: Epithelial Growth Mode: Adherent Karyotype: Aneuploid GMO Status: Genetically Modified Organism Class 1 (GMO1) Subculture Routine: Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2;37°C. Culture Medium: RPMI 1640 + 2mM Glutamine + 6 -10% Foetal Bovine Serum (FBS). Country: FRANCE References: J Urol 1991;143:881; Int J Oncol 1995;6; Int J Cancer 1995;62:724, Biochem Pharm 1999;58: 279-284
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
Cell Line Name: PNT1A Keywords: Human post pubertal prostate normal, immortalised with SV40 Cell Line Description: Established by immortalisation of normal adult prostatic epithelial cells by transfection with a plasmid containing SV40 genome with a defective replication origin. The primary culture was obtained from the prostate of a 35 year old male at post mortem. The cells contain the SV40 genome and express large T protein. They present the phenotype of differentiated luminal prostatic cells with the expression of cytokeratin 8 and 18 (markers for luminal glandular epithelia) and vimentin. Cytokeratin14, a marker of epithelial basal cells, is not expressed. PNT1A cells are non-tumourigenic in nude mice. Species: Human Tissue of Origin: prostate CellType: Epithelial Growth Mode: Adherent Karyotype: Aneuploid GMO Status: Genetically Modified Organism Class 1 (GMO1) Subculture Routine: Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2;37°C. Culture Medium: RPMI 1640 + 2mM Glutamine + 6 -10% Foetal Bovine Serum (FBS). Country: FRANCE References: J Urol 1991;143:881; Int J Oncol 1995;6; Int J Cancer 1995;62:724, Biochem Pharm 1999;58: 279-284
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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