CCD-18Co cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥29865
- 货 号：CCD-18Co cell line细胞株
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
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地址：北京
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CCD-18Co cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
Organism Homo sapiens, human Tissue colonProduct Format frozen Morphology fibroblast Culture Properties adherent Biosafety Level 1 Disease normal Age 2.5 months Gender female Ethnicity Black Clinical Data 2.5 monthsBlackfemaleComments The line begins to senesce at about PDL = 42.Images: Complete Growth Medium The base medium for this cell line is Eagle's Minimum Essential Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Subculturing Remove medium, and rinse with 0.25% trypsin, 0.053 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommendedMedium Renewal: Every 2 to 3 daysNote: Growth of the cells is enhanced by addition of tumor necrosis factor alpha (TNF alpha) to the medium.Cryopreservation Freeze medium: Complete growth medium 95%; DMSO, 5%Storage temperature: liquid nitrogen vapor temperatureCulture Conditions Temperature: 37°CSTR Profile Amelogenin: XCSF1PO: 8D13S317: 12D16S539: 12,13D5S818: 12D7S820: 8TH01: 6,7TPOX: 8,11vWA: 15,17Population Doubling Time The line begins to senesce at about PDL = 42. References Sugarman BJ, et al. Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro. Science 230: 943-945, 1985. PubMed: 3933111
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
Organism Homo sapiens, human Tissue colonProduct Format frozen Morphology fibroblast Culture Properties adherent Biosafety Level 1 Disease normal Age 2.5 months Gender female Ethnicity Black Clinical Data 2.5 monthsBlackfemaleComments The line begins to senesce at about PDL = 42.Images: Complete Growth Medium The base medium for this cell line is Eagle's Minimum Essential Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Subculturing Remove medium, and rinse with 0.25% trypsin, 0.053 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommendedMedium Renewal: Every 2 to 3 daysNote: Growth of the cells is enhanced by addition of tumor necrosis factor alpha (TNF alpha) to the medium.Cryopreservation Freeze medium: Complete growth medium 95%; DMSO, 5%Storage temperature: liquid nitrogen vapor temperatureCulture Conditions Temperature: 37°CSTR Profile Amelogenin: XCSF1PO: 8D13S317: 12D16S539: 12,13D5S818: 12D7S820: 8TH01: 6,7TPOX: 8,11vWA: 15,17Population Doubling Time The line begins to senesce at about PDL = 42. References Sugarman BJ, et al. Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro. Science 230: 943-945, 1985. PubMed: 3933111
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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