pMRE-Tn7-15X plasmid vector质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥39865
- 货 号：pMRE-Tn7-15X plasmid vector质粒载体
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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pMRE-Tn7-15X plasmid vector质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心
pMRE-Tn7-13X Tn7 transposon, Amp R, Cam R； pMRE-Tn7-14X Tn7 transposon, Amp R , Cam R , Gent R ；pMRE-Tn7-15X Tn7 transposon, Amp R , Cam R , Kan R(A) Construction of the pMRE plasmid series. To obtain the pMRE13X plasmid series, HindIII-digestedpPROBE-derived pFru97 was isothermally assembled with a fragment containing the ntpII promoter (PntpII) and a fragment carrying one of eight fluorescent proteingenes (FP) – mTagBFP2, mTurquoise2, sGFP2, mClover3, sYFP2, mOrange2, mScarlet-I, and mCardinal. The rest of the pMRE series were constructed through theassembly of SacI-digested pMRE13X and one of three additional antibiotic resistance gene (AR) – Gentamicin (GentR), Kanamycin (KanR), and Tetracycline (TetR).(B) Construction of the transposon delivery plasmid series pMRE-Tn5 and pMRE-Tn7. PCR products using the pMRE plasmid series as templates containing AR,FP and a Chloramphenicol resistance (CamR) were isothermally inserted into EcoRV digested pAG408 to yield the pMRE-Tn5 series. Similarly, PCR productscontaining AR, FP and a Chloramphenicol resistance (CamR) were blunt-end cloned into SmaI digested pGRG36 to yield the pMRE-Tn7 series.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
pMRE-Tn7-13X Tn7 transposon, Amp R, Cam R； pMRE-Tn7-14X Tn7 transposon, Amp R , Cam R , Gent R ；pMRE-Tn7-15X Tn7 transposon, Amp R , Cam R , Kan R(A) Construction of the pMRE plasmid series. To obtain the pMRE13X plasmid series, HindIII-digestedpPROBE-derived pFru97 was isothermally assembled with a fragment containing the ntpII promoter (PntpII) and a fragment carrying one of eight fluorescent proteingenes (FP) – mTagBFP2, mTurquoise2, sGFP2, mClover3, sYFP2, mOrange2, mScarlet-I, and mCardinal. The rest of the pMRE series were constructed through theassembly of SacI-digested pMRE13X and one of three additional antibiotic resistance gene (AR) – Gentamicin (GentR), Kanamycin (KanR), and Tetracycline (TetR).(B) Construction of the transposon delivery plasmid series pMRE-Tn5 and pMRE-Tn7. PCR products using the pMRE plasmid series as templates containing AR,FP and a Chloramphenicol resistance (CamR) were isothermally inserted into EcoRV digested pAG408 to yield the pMRE-Tn5 series. Similarly, PCR productscontaining AR, FP and a Chloramphenicol resistance (CamR) were blunt-end cloned into SmaI digested pGRG36 to yield the pMRE-Tn7 series.
Supplier来源:BioVector NTCC Inc.
TEL电话:+86-010-53513060
Website网址: http://www.biovector.net
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